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Literature summary for 3.1.1.7 extracted from

  • Shi, J.; Boyd, A.E.; Radic, Z.; Taylor, P.
    Reversibly bound and covalently attached ligands induce conformational changes in the omega loop, Cys69-Cys96, of mouse acetylcholinesterase (2001), J. Biol. Chem., 276, 42196-42204.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
truncated form terminated at position 548, expressed in HEK-293 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
A262C kinetics similar to wild-type enzyme, association and dissociation rate of fasciculin similar to wild type enzyme Mus musculus
E81C kinetics similar to wild-type enzyme Mus musculus
E84C 4fold increased Km, 2fold increase in Kd for edrophonium binding, 18fold increase for 1,5-bis(4-allyldimethylammoniumphenyl)-pentan-3-one dibromide binding Mus musculus
H287C kinetics similar to wild-type enzyme, association and dissociation rate of fasciculin similar to wild type enzyme Mus musculus
L76C kinetics similar to wild-type enzyme Mus musculus
Y124C kinetics similar to wild-type enzyme, association and dissociation rate of fasciculin similar to wild type enzyme Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
1,5-bis(4-allyldimethylammoniumphenyl)-pentan-3-one dibromide
-
Mus musculus
edrophonium
-
Mus musculus
fasciculin
-
Mus musculus
O-O-dimethyl-O-(2,2-dichlorovinyl)phosphate phosphorylates the active site serine Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
acetylthiocholine iodide wild-type enzyme Mus musculus
0.057
-
acetylthiocholine iodide E81C mutant Mus musculus
0.058
-
acetylthiocholine iodide H287C mutant Mus musculus
0.059
-
acetylthiocholine iodide A262C mutant Mus musculus
0.065
-
acetylthiocholine iodide Y124C mutant Mus musculus
0.097
-
acetylthiocholine iodide L76C mutant Mus musculus
0.19
-
acetylthiocholine iodide E84C mutant Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylthiocholine iodide + H2O
-
Mus musculus thiocholine iodide + acetate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2330
-
acetylthiocholine iodide Y124C mutant Mus musculus
2333
-
acetylthiocholine iodide Y124C mutant Mus musculus
2667
-
acetylthiocholine iodide wild-type enzyme Mus musculus
2667
-
acetylthiocholine iodide A262C mutant Mus musculus
2667
-
acetylthiocholine iodide E81C mutant Mus musculus
2670
-
acetylthiocholine iodide wild-type enzyme Mus musculus
2670
-
acetylthiocholine iodide A262C mutant Mus musculus
2670
-
acetylthiocholine iodide E81C mutant Mus musculus
3000
-
acetylthiocholine iodide H287C mutant Mus musculus
3000
-
acetylthiocholine iodide L76C mutant Mus musculus
3167
-
acetylthiocholine iodide E84C mutant Mus musculus
3170
-
acetylthiocholine iodide E84C mutant Mus musculus