Cloned (Comment) | Organism |
---|---|
truncated form terminated at position 548, expressed in HEK-293 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
A262C | kinetics similar to wild-type enzyme, association and dissociation rate of fasciculin similar to wild type enzyme | Mus musculus |
E81C | kinetics similar to wild-type enzyme | Mus musculus |
E84C | 4fold increased Km, 2fold increase in Kd for edrophonium binding, 18fold increase for 1,5-bis(4-allyldimethylammoniumphenyl)-pentan-3-one dibromide binding | Mus musculus |
H287C | kinetics similar to wild-type enzyme, association and dissociation rate of fasciculin similar to wild type enzyme | Mus musculus |
L76C | kinetics similar to wild-type enzyme | Mus musculus |
Y124C | kinetics similar to wild-type enzyme, association and dissociation rate of fasciculin similar to wild type enzyme | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,5-bis(4-allyldimethylammoniumphenyl)-pentan-3-one dibromide | - |
Mus musculus | |
edrophonium | - |
Mus musculus | |
fasciculin | - |
Mus musculus | |
O-O-dimethyl-O-(2,2-dichlorovinyl)phosphate | phosphorylates the active site serine | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
acetylthiocholine iodide | wild-type enzyme | Mus musculus | |
0.057 | - |
acetylthiocholine iodide | E81C mutant | Mus musculus | |
0.058 | - |
acetylthiocholine iodide | H287C mutant | Mus musculus | |
0.059 | - |
acetylthiocholine iodide | A262C mutant | Mus musculus | |
0.065 | - |
acetylthiocholine iodide | Y124C mutant | Mus musculus | |
0.097 | - |
acetylthiocholine iodide | L76C mutant | Mus musculus | |
0.19 | - |
acetylthiocholine iodide | E84C mutant | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylthiocholine iodide + H2O | - |
Mus musculus | thiocholine iodide + acetate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2330 | - |
acetylthiocholine iodide | Y124C mutant | Mus musculus | |
2333 | - |
acetylthiocholine iodide | Y124C mutant | Mus musculus | |
2667 | - |
acetylthiocholine iodide | wild-type enzyme | Mus musculus | |
2667 | - |
acetylthiocholine iodide | A262C mutant | Mus musculus | |
2667 | - |
acetylthiocholine iodide | E81C mutant | Mus musculus | |
2670 | - |
acetylthiocholine iodide | wild-type enzyme | Mus musculus | |
2670 | - |
acetylthiocholine iodide | A262C mutant | Mus musculus | |
2670 | - |
acetylthiocholine iodide | E81C mutant | Mus musculus | |
3000 | - |
acetylthiocholine iodide | H287C mutant | Mus musculus | |
3000 | - |
acetylthiocholine iodide | L76C mutant | Mus musculus | |
3167 | - |
acetylthiocholine iodide | E84C mutant | Mus musculus | |
3170 | - |
acetylthiocholine iodide | E84C mutant | Mus musculus |