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Literature summary for 3.1.1.7 extracted from

  • Walsh, S.B.; Dolden, T.A.; Moores, G.D.; Kristensen, M.; Lewis, T.; Devonshire, A.L.; Williamson, M.S.
    Identification and characterization of mutations in housefly (Musca domestica) acetylcholinesterase involved in insecticide resistance (2001), Biochem. J., 359, 175-181.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.7

Cloned(Commentary)

Cloned (Comment) Organism
-
 Musca domestica

Protein Variants

Protein Variants Comment Organism
F327Y mutation results in resistance to insecticides, the addition of G365A, G262A, G262V and V180L mutation has an additive effect Musca domestica
G262A mutation results in resistance to insecticides, the addition of G365A, G262V, F327Y and V180L mutation has an additive effect Musca domestica
G262A/F327Y mutation results in resistance to insecticides, the addition of G365A, G262V, and V180L mutation has an additive effect Musca domestica
G262V mutation results in strong resistance to insecticides, the addition of G365A, G262A, F327Y and V180L mutation has an additive effect Musca domestica
G262V/F327Y mutation results in resistance to insecticides, the addition of G365A, G262A, G262V, F327Y and V180L mutation has an additive effect Musca domestica
G365A mutation results in resistance to insecticides, the addition of G262A, G262V, F327Y and V180L mutation has an additive effect Musca domestica
V180L mutation results in resistance to insecticides, the addition of G365A, G262A, G262V and F327Y mutation has an additive effect Musca domestica
V180L/F327Y mutation results in resistance to insecticides, the addition of G365A, G262A and G262V mutation has an additive effect Musca domestica
V180L/G262A mutation results in resistance to insecticides, the addition of G365A, G262V and F327Y mutation has an additive effect Musca domestica
V180L/G262A/F327Y mutation results in resistance to insecticides, the addition of G365A and G262V mutation has an additive effect Musca domestica

Inhibitors

Inhibitors Comment Organism Structure
azamethiphos insecticide Musca domestica
bendiocarb insecticide Musca domestica
dichlorvos insecticide Musca domestica
malaoxon insecticide Musca domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 acetylthiocholine iodide F327Y mutant, 25°C, pH 7.5 Musca domestica
0.056
-
 acetylthiocholine iodide G262A/F327Y mutant, 25°C, pH 7.5 Musca domestica
0.075
-
 acetylthiocholine iodide V180L mutant, 25°C, pH 7.5 Musca domestica
0.084
-
 acetylthiocholine iodide V180L/F327Y mutant, 25°C, pH 7.5 Musca domestica
0.129
-
 acetylthiocholine iodide V180L/G262A/F327Y mutant, 25°C, pH 7.5 Musca domestica
0.15
-
 acetylthiocholine iodide wild-type enzyme, 25°C, pH 7.5 Musca domestica
0.153
-
 acetylthiocholine iodide V180L/G262A mutant, 25°C, pH 7.5 Musca domestica
0.205
-
 acetylthiocholine iodide G262A mutant, 25°C, pH 7.5 Musca domestica
0.21
-
 acetylthiocholine iodide G365A mutant, 25°C, pH 7.5 Musca domestica
0.267
-
 acetylthiocholine iodide G262V/F327Y mutant, 25°C, pH 7.5 Musca domestica
1.8
-
 acetylthiocholine iodide G262V mutant, 25°C, pH 7.5 Musca domestica

Organism

Organism UniProt Comment Textmining
Musca domestica Q95P20 housefly, variants insensitive to organophosphate and carbmate insecticides
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylthiocholine iodide + H2O
-
 Musca domestica thiocholine iodide + acetate
-
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