Any feedback?
Literature summary for 3.1.1.6 extracted from
- Biochemical and thermostability features of acetyl esterase Aes from Escherichia coli (2007), Protein Pept. Lett., 14, 165-169.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of wild-type and mutant enzymes in strain BL21(DE3) | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | random mutagenesis and screening for thermostable mutants at 85°C | Escherichia coli |
| T74A | random mutagenesis, the mutant shows increased thermostability compared to the wild-type enzyme | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.245 | - |
4-nitrophenyl hexanoate | pH 7.1, 65°C, recombinant wild-type enzyme | Escherichia coli |  |
| 0.355 | - |
4-nitrophenyl hexanoate | pH 7.1, 65°C, recombinant mutant T74A | Escherichia coli | |
| 0.75 | - |
4-nitrophenyl-butanoate | pH 7.1, 25°C, recombinant wild-type enzyme | Escherichia coli | |
| 1.5 | - |
4-nitrophenyl-butanoate | pH 7.1, 65°C, recombinant wild-type enzyme | Escherichia coli | |
| 2 | - |
4-nitrophenyl-butanoate | pH 7.1, 65°C, recombinant mutant T74A | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography, and gel filtration | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 250 | - |
pH 7.1, 65°C, purified recombinant wild-type enzyme | Escherichia coli |
| 270 | - |
pH 7.1, 65°C, purified recombinant mutant T74A | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl hexanoate + H2O | - |
Escherichia coli | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl-butanoate + H2O | - |
Escherichia coli | 4-nitrophenol + butanoate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetyl-esterase | - |
Escherichia coli |
| Aes | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 75 | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
t1/2 inactivation is 5 min for the wild-type enzyme, and 30 min for mutant T74A, thermal inactivation first-order kinetics, overview | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21 | - |
4-nitrophenyl-butanoate | pH 7.1, 25°C, recombinant wild-type enzyme | Escherichia coli | |
| 34.5 | - |
4-nitrophenyl hexanoate | pH 7.1, 65°C, recombinant wild-type enzyme | Escherichia coli | |
| 48.8 | - |
4-nitrophenyl hexanoate | pH 7.1, 65°C, recombinant mutant T74A | Escherichia coli | |
| 145 | - |
4-nitrophenyl-butanoate | pH 7.1, 65°C, recombinant wild-type enzyme | Escherichia coli | |
| 155 | - |
4-nitrophenyl-butanoate | pH 7.1, 65°C, recombinant mutant T74A | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
- |
Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
