Application | Comment | Organism |
---|---|---|
drug development | drug design, two essential pharmacophoric groups of ligand: 9-O-acetyl group and C-1 carboxylate group, anti-virus infection | bovine coronavirus |
drug development | drug design, two essential pharmacophoric groups of ligand: 9-O-acetyl group and C-1 carboxylate group, anti-virus infection | influenza C virus |
Crystallization (Comment) | Organism |
---|---|
molecular modelling using PDB: 1FLC, and alpha-methyl or beta-methyl glycosides of 9-acetamido-9-deoxy-N-acetylneuraminic acid or N-acetyl-9-O-acetyl-2,6-alpha-S-glucosyl-neuraminic acid alpha-methylglycoside as substrates, no interaction between aglycon moiety and the enzyme or its active site, catalytic triad: Ser57, His355, Asp352, catalysis involves conformational change of Ser57 side chain in order to enable 9-O-acetyl-binding and position for nucleophilic attack on 9-O-acetate carbonyl carbon, beta-anomer substrate lacks bi-dendate interaction between C-1 carboxylate group and Asp352 and is no suitable substrate | influenza C virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | bovine coronavirus | alpha-anomer specificity | ? | - |
? | |
additional information | influenza C virus | alpha-anomer specificity | ? | - |
? | |
additional information | influenza C virus | Ser57 is essential for substrate binding and orientation and catalytic activity, NMR-analyses and molecular modelling | ? | - |
? | |
additional information | influenza C virus Johannesburg | alpha-anomer specificity | ? | - |
? | |
additional information | influenza C virus Johannesburg | Ser57 is essential for substrate binding and orientation and catalytic activity, NMR-analyses and molecular modelling | ? | - |
? | |
additional information | bovine coronavirus BcoV | alpha-anomer specificity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
bovine coronavirus | - |
BcoV | - |
bovine coronavirus BcoV | - |
BcoV | - |
influenza C virus | P07975 | INF-C | - |
influenza C virus Johannesburg | P07975 | INF-C | - |
Purification (Comment) | Organism |
---|---|
from baculovirus infected insect Sf9 cells as GFP-fusion protein by ultracentrifugation, ion-exchange chromatography on Q-Sepharose FF column and dialysis | influenza C virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl 6-O-(N-acetyl-9-O-acetyl-alpha-D-neuramin-2-yl)-alpha-D-glucoside + H2O | methyl N-acetylneuraminide, 25°C, 60 min, pH 7 | bovine coronavirus | methyl 6-O-(N-acetyl-alpha-D-neuramin-2-yl)-alpha-D-glucoside + acetate | NMR-based enzyme assay | ? | |
methyl 6-S-(N-acetyl-9-O-acetyl-alpha-D-neuramin-2-yl)-6-thio-alpha-D-glucoside + H2O | thio-linked N-acetylneuraminosylglucoside, 25°C, 60 min, pH 7 | bovine coronavirus | methyl 6-S-(N-acetyl-alpha-D-neuramin-2-yl)-alpha-D-glucoside + acetate | NMR-based enzyme assay | ? | |
methyl 6-S-(N-acetyl-9-O-acetyl-alpha-D-neuramin-2-yl)-6-thio-alpha-D-glucoside + H2O | thio-linked N-acetylneuraminosylglucoside, 25°C, 60 min, pH 7 | influenza C virus | methyl 6-S-(N-acetyl-alpha-D-neuramin-2-yl)-alpha-D-glucoside + acetate | NMR-based enzyme assay | ? | |
methyl 6-S-(N-acetyl-9-O-acetyl-alpha-D-neuramin-2-yl)-6-thio-alpha-D-glucoside + H2O | thio-linked N-acetylneuraminosylglucoside, 25°C, 60 min, pH 7 | bovine coronavirus BcoV | methyl 6-S-(N-acetyl-alpha-D-neuramin-2-yl)-alpha-D-glucoside + acetate | NMR-based enzyme assay | ? | |
additional information | alpha-anomer specificity | bovine coronavirus | ? | - |
? | |
additional information | alpha-anomer specificity | influenza C virus | ? | - |
? | |
additional information | Ser57 is essential for substrate binding and orientation and catalytic activity, NMR-analyses and molecular modelling | influenza C virus | ? | - |
? | |
additional information | enzyme activity independent of chemical nature of aglycon moiety | influenza C virus | ? | - |
? | |
additional information | specific for alpha-anomer of N-acetyl-9-O-acetyl-neuraminic acid, N-acetyl-9-O-acetyl-neuraminic acid beta-methylglycoside is no substrate (60 min, 25°C, pH 7) | bovine coronavirus | ? | - |
? | |
additional information | specific for alpha-anomer of N-acetyl-9-O-acetyl-neuraminic acid, N-acetyl-9-O-acetyl-neuraminic acid beta-methylglycoside is no substrate (60 min, 25°C, pH 7) | influenza C virus | ? | - |
? | |
additional information | substrate specificity towards aglycon moiety | bovine coronavirus | ? | - |
? | |
additional information | alpha-anomer specificity | influenza C virus Johannesburg | ? | - |
? | |
additional information | Ser57 is essential for substrate binding and orientation and catalytic activity, NMR-analyses and molecular modelling | influenza C virus Johannesburg | ? | - |
? | |
additional information | enzyme activity independent of chemical nature of aglycon moiety | influenza C virus Johannesburg | ? | - |
? | |
additional information | specific for alpha-anomer of N-acetyl-9-O-acetyl-neuraminic acid, N-acetyl-9-O-acetyl-neuraminic acid beta-methylglycoside is no substrate (60 min, 25°C, pH 7) | influenza C virus Johannesburg | ? | - |
? | |
additional information | alpha-anomer specificity | bovine coronavirus BcoV | ? | - |
? | |
additional information | specific for alpha-anomer of N-acetyl-9-O-acetyl-neuraminic acid, N-acetyl-9-O-acetyl-neuraminic acid beta-methylglycoside is no substrate (60 min, 25°C, pH 7) | bovine coronavirus BcoV | ? | - |
? | |
additional information | substrate specificity towards aglycon moiety | bovine coronavirus BcoV | ? | - |
? | |
N-acetyl-9-O-acetyl-neuraminic acid + H2O | 25°C, 60 min, pH 7, slow activity due to alpha: beta anomeric ratio of about 5: 95 | bovine coronavirus | N-acetylneuraminic acid + acetate | NMR-based enzyme assay | ? | |
N-acetyl-9-O-acetyl-neuraminic acid + H2O | 25°C, 60 min, pH 7, slow activity due to alpha: beta anomeric ratio of about 5:95 | influenza C virus | N-acetylneuraminic acid + acetate | NMR-based enzyme assay | ? | |
N-acetyl-9-O-acetyl-neuraminic acid alpha-methylglycoside + H2O | methyl N-acetylneuraminide, 25°C, 60 min, pH 7 | influenza C virus | N-acetylneuraminic acid alpha-methylglycoside + acetate | NMR-based enzyme assay | ? | |
p-nitrophenyl acetate + H2O | - |
bovine coronavirus | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl acetate + H2O | - |
influenza C virus | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl acetate + H2O | - |
influenza C virus Johannesburg | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl acetate + H2O | - |
bovine coronavirus BcoV | p-nitrophenol + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
haemagglutinin esterase | - |
bovine coronavirus |
haemagglutinin esterase fusion | - |
influenza C virus |
HE | - |
bovine coronavirus |
HEF | - |
influenza C virus |
INF-C esterase | - |
influenza C virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | methyl-linked N-acetyl-neuraminide (N-acetyl-9-O-acetyl-neuraminic acid alpha-methylglycoside) and thio-linked N-acetylneuraminosylglucoside (N-acetyl-9-O-acetyl-2,6-alpha-S-glucosyl-neuraminic acid alpha-methylglycoside) hydrolysed at similar rates | influenza C virus | |
additional information | - |
additional information | methyl-linked N-acetyl-neuraminide (N-acetyl-9-O-acetyl-neuraminic acid alpha-methylglycoside) hydrolysed at higher rate than thio-linked N-acetylneuraminosylglucoside (N-acetyl-9-O-acetyl-2,6-alpha-S-glucosyl-neuraminic acid alpha-methylglycoside) | bovine coronavirus |