Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Alpha-naphthyl acetate | competitive inhibitor | Rattus norvegicus | |
Cu2+ | complete inhibition at 1 mM | Rattus norvegicus | |
diisopropyl fluorophosphates | complete inhibition at 1 mM | Rattus norvegicus | |
Hg2+ | complete inhibition at 1 mM | Rattus norvegicus | |
phenylmethylsulfonyl fluoride | complete inhibition at 10 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.652 | - |
spironolactone | pH 7.5, 37°C | Rattus norvegicus | |
1.28 | - |
acetyl-CoA | pH 7.5, 37°C | Rattus norvegicus | |
1.71 | - |
Alpha-naphthyl acetate | pH 7.5, 37°C | Rattus norvegicus | |
22 | - |
alacepril | pH 7.5, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
microsome | only little activity | Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
1 * 29000 + 1 * 36000, SDS-PAGE | Rattus norvegicus |
36000 | - |
1 * 29000 + 1 * 36000, SDS-PAGE | Rattus norvegicus |
60000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Sprague-Dawley | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.695 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O | - |
Rattus norvegicus | CoA + acetate | - |
? | |
alacepril + H2O | - |
Rattus norvegicus | deacetylalacepril + acetate | - |
? | |
alpha-naphthyl acetate + H2O | - |
Rattus norvegicus | alpha-naphthol + acetate | - |
? | |
spironolactone + H2O | - |
Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 29000 + 1 * 36000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
room temperature, complete inactivation in the presence of 10 mM 2-mercaptoethanol after 8 h | Rattus norvegicus |