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Literature summary for 3.1.1.50 extracted from

  • Bogevik, A.S.; Tocher, D.R.; Waagboe, R.; Olsen, R.E.
    Triacylglycerol-, wax ester- and sterol ester-hydrolases in midgut of Atlantic salmon (Salmo salar) (2008), Aquac. Nutr., 14, 93-98.
    View publication on PubMed
Search for more literature for 3.1.1.50

Activating Compound

Activating Compound Comment Organism Structure
taurocholate 20 mM Oncorhynchus mykiss
taurocholate 20 mM Salmo salar

General Stability

General Stability Organism
activity measurable after 4 h at 10°C Oncorhynchus mykiss
activity measurable after 4 h at 10°C Salmo salar

Organism

Organism UniProt Comment Textmining
Oncorhynchus mykiss
-
-
-
Salmo salar
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
intestine crude intestine extract, mid-gut, 10-12 h after feeding Oncorhynchus mykiss
-
intestine crude intestine extract, mid-gut, 10-12 h after feeding Salmo salar
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 linearly increasing activity up to 24 h of incubation Salmo salar
additional information
-
 linearly increasing activity with increasing protein concentration up to 200 microgram protein in the assay Salmo salar
additional information
-
 linearly increasing activity with increasing substrate concentration, steep increase up to 125 microM oleic acid Salmo salar
0.000072
-
 crude mid-gut extracts, desalted Salmo salar
0.000254
-
 crude mid-gut extracts Salmo salar
0.000278
-
 crude mid-gut extracts, desalted Oncorhynchus mykiss
0.000305
-
 crude mid-gut extracts Oncorhynchus mykiss
0.000625
-
 crude mid-gut extracts, desalted, in presence of 20 mM taurocholate Oncorhynchus mykiss
0.00063
-
 crude mid-gut extracts, desalted, in presence of 20 mM taurocholate and trypsin inhibitor Salmo salar
0.000665
-
 crude mid-gut extracts, desalted, in presence of 20 mM taurocholate Salmo salar

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmityl oleate + H2O 4 h, 10°C, in presence or absence of 20 mM bile salt (taurocholate) Oncorhynchus mykiss hexadecanol + oleic acid reaction stop with chloroform/methanol/toluene mixture ?
palmityl oleate + H2O 4 h, 10°C, in presence or absence of 20 mM bile salt (taurocholate) Salmo salar hexadecanol + oleic acid reaction stop with chloroform/methanol/toluene mixture ?

Synonyms

Synonyms Comment Organism
wax ester hydrolase
-
 Oncorhynchus mykiss
wax ester hydrolase
-
 Salmo salar
WEH
-
 Oncorhynchus mykiss
WEH
-
 Salmo salar