Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sodium deoxycholate | 0.1%, strongly stimulates phospholipase B activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | - |
Saccharomyces cerevisiae | |
deoxycholate | - |
Saccharomyces cerevisiae | |
Fe3+ | - |
Saccharomyces cerevisiae | |
Hg2+ | 5 mM, inhibits phospholipase B activity by 67%, no inhibition of lysophospholipase activity | Saccharomyces cerevisiae | |
SDS | - |
Saccharomyces cerevisiae | |
Triton X-100 | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
phosphatidylcholine | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | tightly bound to membrane | Saccharomyces cerevisiae | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
330000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | - |
Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
phospholipase B activity | Saccharomyces cerevisiae |
193 | - |
lysophospholipase activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-acyl-sn-glycero-3-phosphocholine + H2O | - |
Saccharomyces cerevisiae | fatty acid + glycero-3-phosphocholine | - |
? | |
2-acylglycerophosphocholine + H2O | 2-acyl-sn-glycero-3-phosphocholine | Saccharomyces cerevisiae | fatty acid + glycerylphosphocholine | - |
? | |
phosphatidylcholine + H2O | phospholipase B activity, hydrolyzes the acyl ester bonds sequentially, first the 2-acyl group and then the 1-acyl group | Saccharomyces cerevisiae | lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | 4 | - |
Saccharomyces cerevisiae |