Any feedback?
Literature summary for 3.1.1.41 extracted from
- A novel cephalosporin deacetylating acetyl xylan esterase from Bacillus subtilis with high activity toward cephalosporin C and 7-aminocephalosporanic acid (2014), Appl. Microbiol. Biotechnol., 98, 2081-2089.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Cah, subcloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21(DE3), the enzyme is secreted | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Bacillus subtilis |  |
| Ba2+ | low inhibition at 1 mM | Bacillus subtilis | |
| Ca2+ | low inhibition at 1 mM | Bacillus subtilis | |
| Cd2+ | low inhibition at 1 mM | Bacillus subtilis | |
| Co2+ | low inhibition at 1 mM | Bacillus subtilis | |
| Cu2+ | strong inhibition at 1 mM | Bacillus subtilis | |
| diethyldicarbonate | - |
Bacillus subtilis | |
| Fe2+ | low inhibition at 1 mM | Bacillus subtilis | |
| Fe3+ | strong inhibition at 1 mM | Bacillus subtilis | |
| Mg2+ | low inhibition at 1 mM | Bacillus subtilis | |
| Mn2+ | low inhibition at 1 mM | Bacillus subtilis | |
| additional information | no inhibition by EDTA and K+ | Bacillus subtilis | |
| PMSF | - |
Bacillus subtilis | |
| SDS | low inhibition at 1 mM | Bacillus subtilis | |
| Zn2+ | strong inhibition at 1 mM | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
6 * 36000, recombinant enzyme, SDS-PAGE | Bacillus subtilis |
| 223000 | - |
recombinant enzyme, gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bacillus subtilis | the enzyme has a double specificity on both the acetylated oligosaccharide, cf. EC 3.1.1.72, and cephalosporin C and 7-aminocephalosporanic acid | ? | - |
? | |
| additional information | Bacillus subtilis CICC 20034 | the enzyme has a double specificity on both the acetylated oligosaccharide, cf. EC 3.1.1.72, and cephalosporin C and 7-aminocephalosporanic acid | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | M1JUH6 | gene Cah | - |
| Bacillus subtilis CICC 20034 | M1JUH6 | gene Cah | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 1.2fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, gel filtration, dialysis, and ultrafiltration | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.72 | - |
purified recombinant enzyme, pH 7.0, 40°C, substrate 4-nitrophenyl butyrate | Bacillus subtilis |
| 741 | - |
purified recombinant enzyme, pH 7.0, 40°C, substrate alpha-naphthyl acetate | Bacillus subtilis |
| 962 | - |
purified recombinant enzyme, pH 7.0, 40°C, substrate beta-naphthyl acetate | Bacillus subtilis |
| 1086 | - |
purified recombinant enzyme, pH 7.0, 40°C, substrate 4-methylumbelliferyl acetate | Bacillus subtilis |
| 1245 | - |
purified recombinant enzyme, pH 7.0, 40°C, substrate glucose pentacetate | Bacillus subtilis |
| 2949 | - |
purified recombinant enzyme, pH 7.0, 40°C, substrate 4-nitrophenyl acetate | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-naphthyl acetate + H2O | - |
Bacillus subtilis | 2-naphthol + acetate | - |
? | |
| 2-naphthyl acetate + H2O | - |
Bacillus subtilis CICC 20034 | 2-naphthol + acetate | - |
? | |
| 3-naphthyl acetate + H2O | - |
Bacillus subtilis | 3-naphthol + acetate | - |
? | |
| 4-methylumbelliferyl acetate + H2O | - |
Bacillus subtilis | 4-methylumbelliferol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Bacillus subtilis | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Bacillus subtilis CICC 20034 | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Bacillus subtilis | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Bacillus subtilis CICC 20034 | 4-nitrophenol + butyrate | - |
? | |
| glucose pentacetate + H2O | - |
Bacillus subtilis | ? | - |
? | |
| additional information | the enzyme has a double specificity on both the acetylated oligosaccharide, cf. EC 3.1.1.72, and cephalosporin C and 7-aminocephalosporanic acid | Bacillus subtilis | ? | - |
? | |
| additional information | the enzyme hydrolyzes the ester linkages of the acetyl groups in both the xylose moieties of the acetylated xylan fragments | Bacillus subtilis | ? | - |
? | |
| additional information | the enzyme has a double specificity on both the acetylated oligosaccharide, cf. EC 3.1.1.72, and cephalosporin C and 7-aminocephalosporanic acid | Bacillus subtilis CICC 20034 | ? | - |
? | |
| additional information | the enzyme hydrolyzes the ester linkages of the acetyl groups in both the xylose moieties of the acetylated xylan fragments | Bacillus subtilis CICC 20034 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 36000, recombinant enzyme, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 75 | activity range, profile overview | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant enzyme, pH 7.0, stable up to, loss of 10% activity after 120 h | Bacillus subtilis |
| 50 | - |
purified recombinant enzyme, pH 7.0, loss of 20% activity after 120 h | Bacillus subtilis |
| 50 | - |
purified recombinant enzyme, pH 7.0, loss of 60% activity after 120 h | Bacillus subtilis |
| 60 | - |
purified recombinant enzyme, pH 7.0, inactivation within 10 h | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | activity range, profile overview | Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 12 | purified recombinant enzyme, over 80% of maximal activity within this range | Bacillus subtilis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to carbohydrate esterase family 7 | Bacillus subtilis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
