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Literature summary for 3.1.1.41 extracted from
- A colorimetric assay for the determination of acetyl xylan esterase or cephalosporin C acetyl esterase activities using 7-amino cephalosporanic acid, cephalosporin C, or acetylated xylan as substrate (2007), Anal. Biochem., 369, 210-217.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | enzyme reaction products deacetyl cephalosporins are a highly valuable starting material for producing semisynthetic beta-lactam antibiotics | Bacillus pumilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene axe, expression of His-tagged enzyme in Escherichia coli | Bacillus pumilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Bacillus pumilus | |
| 215 | - |
cephalosporin C | pH 7.3, 25°C, recombinant enzyme | Bacillus pumilus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus pumilus | - |
gene axe | - |
| Bacillus pumilus CECT 5072 | - |
gene axe | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Bacillus pumilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-aminocephalosporanic acid + H2O | colorimetric assay method development using bromothymol blue, validation, overview | Bacillus pumilus | 7-amino-3-deacetylcephalosporanic acid + acetate | - |
? | |
| 7-aminocephalosporanic acid + H2O | colorimetric assay method development using bromothymol blue, validation, overview | Bacillus pumilus CECT 5072 | 7-amino-3-deacetylcephalosporanic acid + acetate | - |
? | |
| cephalosporin C + H2O | colorimetric assay method development using bromothymol blue, validation, overview | Bacillus pumilus | deacetylcephalosporin C + acetate | - |
? | |
| cephalosporin C + H2O | colorimetric assay method development using bromothymol blue, validation, overview | Bacillus pumilus CECT 5072 | deacetylcephalosporin C + acetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetyl xylan esterase | - |
Bacillus pumilus |
| AXE | - |
Bacillus pumilus |
| cephalosporin C acetyl esterase | - |
Bacillus pumilus |
| cephalosporin C deacetylase | - |
Bacillus pumilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bacillus pumilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
enzymatic hydrolysis around pH 7.0 is the most effective method for preparing deacetyl derivatives of cephalosporin | Bacillus pumilus |
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Release 2023.1 (January 2023)
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