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Literature summary for 3.1.1.41 extracted from
- High-level expression, purification, and some properties of a recombinant cephalosporin-C deacetylase (1999), J. Biosci. Bioeng., 87, 456-462.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus subtilis |
| the most efficient expression plasmid is pCAH431, the expression and purification system has made the industrial production of the enzyme possible | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.4 | - |
7-aminocephalosporanic acid | recombinant enzyme, 30°C | Bacillus subtilis |  |
| 9.2 | - |
cephaloglycin | recombinant enzyme | Bacillus subtilis | |
| 11.7 | - |
cephalotin | recombinant enzyme | Bacillus subtilis | |
| 16.7 | - |
cephapirin | recombinant enzyme | Bacillus subtilis | |
| 34.5 | - |
cephalosporin C | recombinant enzyme | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
8 * 35000, strain SHS 0133, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis SHS 0133 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme, the expression and purification system has made the industrial production of the enzyme possible | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 210 | - |
- |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for 1 year | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-aminocephalosporanic acid + H2O | - |
Bacillus subtilis | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
| 7-aminocephalosporanic acid + H2O | - |
Bacillus subtilis SHS 0133 | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
| cephaloglycin + H2O | - |
Bacillus subtilis | acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid | - |
? | |
| cephaloglycin + H2O | - |
Bacillus subtilis SHS 0133 | acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid | - |
? | |
| cephalosporin C + H2O | - |
Bacillus subtilis | deacetylcephalosporin C + acetate | - |
? | |
| cephalothin + H2O | - |
Bacillus subtilis | acetate + deacetylcephalothin | - |
? | |
| cephalothin + H2O | - |
Bacillus subtilis SHS 0133 | acetate + deacetylcephalothin | - |
? | |
| cephapirin + H2O | - |
Bacillus subtilis | acetate + deacetylcephapirin | - |
? | |
| cephapirin + H2O | - |
Bacillus subtilis SHS 0133 | acetate + deacetylcephapirin | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Bacillus subtilis | 4-nitrophenol + acetate | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Bacillus subtilis SHS 0133 | 4-nitrophenol + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 35000, strain SHS 0133, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CAH | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Bacillus subtilis |
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