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Literature summary for 3.1.1.41 extracted from
- Purification and properties of cephalosporin-C deacetylase from the yeast, Rhodotorula glutinis 38B1, useful for bioconversion of 7-aminocephalosporanic acid derivatives (1998), J. Ferment. Bioeng., 85, 53-57.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (p-amino-phenyl)methanesulfonyl fluoride | slight inhibition | Rhodotorula glutinis |  |
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Rhodotorula glutinis | |
| Cu2+ | CuCl2 | Rhodotorula glutinis | |
| eserine sulfate | slight inhibition | Rhodotorula glutinis | |
| Hg2+ | HgCl2 | Rhodotorula glutinis | |
| PbCl2 | - |
Rhodotorula glutinis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.11 | - |
benzyloxycarbonyl-7-aminocephalosporanic acid | - |
Rhodotorula glutinis | |
| 1.43 | - |
7-aminocephalosporanic acid | - |
Rhodotorula glutinis | |
| 2.03 | - |
monochloroacetyl-7-aminocephalosporanic acid | - |
Rhodotorula glutinis | |
| 8.39 | - |
cephalosporin C | - |
Rhodotorula glutinis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | the majority of the enzyme is tightly bound to the cell wall | Rhodotorula glutinis | 5618 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 82000 | - |
2 * 82000, SDS-PAGE | Rhodotorula glutinis |
| 141000 | - |
gel filtration | Rhodotorula glutinis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula glutinis | - |
- |
- |
| Rhodotorula glutinis 38B1 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | N-linked sugar content is about 21% by weight | Rhodotorula glutinis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodotorula glutinis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 342 | - |
- |
Rhodotorula glutinis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-aminocephalosporanic acid + H2O | - |
Rhodotorula glutinis | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
| 7-aminocephalosporanic acid + H2O | - |
Rhodotorula glutinis 38B1 | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
| benzyloxycarbonyl-7-aminocephalosporanic acid + H2O | - |
Rhodotorula glutinis | ? | - |
? | |
| benzyloxycarbonyl-7-aminocephalosporanic acid + H2O | - |
Rhodotorula glutinis 38B1 | ? | - |
? | |
| cephalosporin C + H2O | - |
Rhodotorula glutinis | deacetylcephalosporin C + acetate | - |
? | |
| cephalosporin C + H2O | - |
Rhodotorula glutinis 38B1 | deacetylcephalosporin C + acetate | - |
? | |
| monochloroacetyl-7-aminocephalosporanic acid + H2O | - |
Rhodotorula glutinis | acetate + monochloroacetyl-deacetyl-7-aminocephalosporanic acid | - |
? | |
| monochloroacetyl-7-aminocephalosporanic acid + H2O | - |
Rhodotorula glutinis 38B1 | acetate + monochloroacetyl-deacetyl-7-aminocephalosporanic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 82000, SDS-PAGE | Rhodotorula glutinis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Rhodotorula glutinis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 45 | 10°C: about 40% of maximal activity, 45°C: about 30% of maximal activity | Rhodotorula glutinis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
15 min, 60% loss of activity | Rhodotorula glutinis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 534 | - |
benzyloxycarbonyl-7-aminocephalosporanic acid | - |
Rhodotorula glutinis | |
| 576 | - |
7-aminocephalosporanic acid | - |
Rhodotorula glutinis | |
| 768 | - |
monochloroacetyl-7-aminocephalosporanic acid | - |
Rhodotorula glutinis | |
| 1370 | - |
cephalosporin C | - |
Rhodotorula glutinis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Rhodotorula glutinis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | 7 | pH 4.2: 30% of maximal activity, pH 7.0: about 35% of maximal activity | Rhodotorula glutinis |
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