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Literature summary for 3.1.1.4 extracted from

  • Ghomashchi, F.; Naika, G.S.; Bollinger, J.G.; Aloulou, A.; Lehr, M.; Leslie, C.C.; Gelb, M.H.
    Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms (2010), J. Biol. Chem., 285, 36100-36111.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.4

Activating Compound

Activating Compound Comment Organism Structure
cardiolipin cytosolic isoform PLA2beta action on phosphatidylcholine vesicles is activated by anionic phosphoinositides and cardiolipin Mus musculus
cardiolipin cytosolic isoform PLA2beta action on phosphatidylcholine vesicles is activated by anionic phosphoinositides and cardiolipin Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Mus musculus
expressed in Sf9 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
arachidonyltrifluoromethyl ketone
-
 Homo sapiens
arachidonyltrifluoromethyl ketone
-
 Mus musculus
pyrrolidine-1 isoform cPLA2alpha inhibitor Homo sapiens
pyrrolidine-1 isoform cPLA2alpha inhibitor Mus musculus
pyrrolidine-2 isoform cPLA2alpha inhibitor Homo sapiens
pyrrolidine-2 isoform cPLA2alpha inhibitor Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-
cytosol
-
 Homo sapiens 5829
-
vesicle
-
 Mus musculus 31982
-
vesicle
-
 Homo sapiens 31982
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ cytosolic isoform PLA2beta action on phosphatidylcholine vesicles displays a requirement for Ca2+ only in the presence of cardiolipin Mus musculus
Ca2+ cytosolic isoform PLA2beta action on phosphatidylcholine vesicles displays a requirement for Ca2+ only in the presence of cardiolipin Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipids + H2O Mus musculus
-
 ?
-
 ?
phospholipids + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Mus musculus
Ni-NTA resin column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000006
-
 isoform cPLA2delta, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C Homo sapiens
0.000015
-
 isoform cPLA2epsilon, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C Homo sapiens
0.000021
-
 isoform cPLA2epsilon, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C Homo sapiens
0.00004
-
 isoform cPLA2epsilon, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C Homo sapiens
0.00006
-
 isoform cPLA2delta, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C Homo sapiens
0.000145
-
 isoform cPLA2epsilon, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C Homo sapiens
0.00021
-
 isoform cPLA2delta, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C Homo sapiens
0.000245
-
 isoform cPLA2beta, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C Mus musculus
0.000295
-
 isoform cPLA2beta, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C Mus musculus
0.0003
-
 isoform cPLA2gamma, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C Homo sapiens
0.0003
-
 isoform cPLA2gamma, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C Homo sapiens
0.0011
-
 isoform cPLA2alpha, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C Homo sapiens
0.00245
-
 isoform cPLA2zeta, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C Mus musculus
0.0225
-
 isoform cPLA2delta, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C Homo sapiens
0.05
-
 isoform cPLA2gamma, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C Homo sapiens
0.058
-
 isoform cPLA2gamma, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C Homo sapiens
0.06
-
 isoform cPLA2zeta, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C Mus musculus
0.126
-
 isoform cPLA2alpha, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C Homo sapiens
0.145
-
 isoform cPLA2alpha, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C Homo sapiens
0.23
-
 isoform cPLA2alpha, using 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C Homo sapiens
0.365
-
 isoform cPLA2beta, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C Mus musculus
0.3695
-
 isoform cPLA2zeta, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, 0.02 mM Ca2+, at 37°C Mus musculus
0.372
-
 isoform cPLA2zeta, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C Mus musculus
0.381
-
 isoform cPLA2beta, using 1-palmitoyl-sn-glycero-3-phosphocholine as substrate, in 10 mM MOPS, pH 7.2, 0.5 mM EGTA, 100 mM KCl, 0.5 mg/ml bovine serum albumin, in the absence of Ca2+, at 37°C Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine + H2O
-
 Mus musculus ?
-
 ?
1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine + H2O
-
 Homo sapiens ?
-
 ?
1-palmitoyl-2-oleoyl-phosphatidylcholine + H2O
-
 Mus musculus ?
-
 ?
1-palmitoyl-2-oleoyl-phosphatidylcholine + H2O
-
 Homo sapiens ?
-
 ?
1-palmitoyl-2-oleoyl-phosphatidylethanolamine + H2O
-
 Mus musculus ?
-
 ?
1-palmitoyl-2-oleoyl-phosphatidylethanolamine + H2O
-
 Homo sapiens ?
-
 ?
1-palmitoyl-2-oleoyl-phosphatidylglycerol + H2O
-
 Mus musculus ?
-
 ?
1-palmitoyl-2-oleoyl-phosphatidylglycerol + H2O
-
 Homo sapiens ?
-
 ?
1-palmitoyl-2-oleoyl-phosphatidylinositol + H2O
-
 Mus musculus ?
-
 ?
1-palmitoyl-2-oleoyl-phosphatidylinositol + H2O
-
 Homo sapiens ?
-
 ?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate + H2O
-
 Mus musculus ?
-
 ?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate + H2O
-
 Homo sapiens ?
-
 ?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
-
 Mus musculus ?
-
 ?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens ?
-
 ?
additional information isoform cPLA2beta shows no activity with 1-palmitoyl-2-oleoyl-phosphatidylcholine Mus musculus ?
-
 ?
additional information isoform cPLA2gamma shows no activity with 1-palmitoyl-2-oleoyl-phosphatidylcholine, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate, 1-palmitoyl-2-oleoyl-phosphatidylethanolamine, 1-palmitoyl-2-oleoyl-phosphatidylglycerol, and 1-palmitoyl-2-oleoyl-phosphatidylinositol Homo sapiens ?
-
 ?
phospholipids + H2O
-
 Mus musculus ?
-
 ?
phospholipids + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
cPLA2alpha isoform Mus musculus
cPLA2alpha isoform Homo sapiens
cPLA2beta isoform Mus musculus
cPLA2beta isoform Homo sapiens
cPLA2delta isoform Homo sapiens
cPLA2epsilon isoform Homo sapiens
cPLA2zeta isoform Mus musculus
group IVB phospholipase A2
-
 Mus musculus
group IVB phospholipase A2
-
 Homo sapiens