Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.4 extracted from

  • Hu, F.; Hu, X.; Ma, C.; Zhao, J.; Xu, J.; Yu, X.
    Molecular characterization of a novel Clonorchis sinensis secretory phospholipase A(2) and investigation of its potential contribution to hepatic fibrosis (2009), Mol. Biochem. Parasitol., 167, 127-134.
    View publication on PubMed
Search for more literature for 3.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
PCR product recombined into the pET-28a expression vector with the coding region in frame with the 6 × His tag, expressed in Escherichia coli BL-21 (DE3) Clonorchis sinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.347
-
 phosphatidylcholine standard PLA2, in 100 mM Tris-HCl buffer, pH 8.5, at 40°C, 50 mM sodium bis(2-ethylhexyl) sulfosuccinate and 0.05mM phenolsulfonphthalein in isooctane synthetic construct
4.847
-
 phosphatidylcholine recombinant group III sPLA2, in 100 mM Tris-HCl buffer, pH 8.5, at 40°C, 50 mM sodium bis(2-ethylhexyl) sulfosuccinate and 0.05mM phenolsulfonphthalein in isooctane Clonorchis sinensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on synthetic construct
Ca2+ dependent on Clonorchis sinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, SDS-PAGE Clonorchis sinensis

Organism

Organism UniProt Comment Textmining
Clonorchis sinensis C6YBD6
-
-
synthetic construct
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by centrifugation and gel filtration Clonorchis sinensis

Source Tissue

Source Tissue Comment Organism Textmining
adult
-
 Clonorchis sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O
-
 synthetic construct 1-acylglycerophosphocholine + a carboxylate
-
 ?
phosphatidylcholine + H2O
-
 Clonorchis sinensis 1-acylglycerophosphocholine + a carboxylate
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Clonorchis sinensis

Synonyms

Synonyms Comment Organism
GIIIsPLA2
-
 Clonorchis sinensis
group III sPLA2
-
 Clonorchis sinensis
phospholipase A2
-
 synthetic construct
PLA2
-
 synthetic construct
secretory phospholipase A2
-
 Clonorchis sinensis

General Information

General Information Comment Organism
physiological function group III sPLA2 may play an important role in the initiation and development of hepatic fibrosis. Expression of collagen III is upregulated in LX-2 cells after incubation with the recombinant protein. Group III sPLA2 can bind to specific sites (specific receptor), not lipids, on LX-2 cells membrane. Group III sPLA2 is a component of excretory-secretory products Clonorchis sinensis