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Literature summary for 3.1.1.4 extracted from

  • Markakis, K.P.; Koropouli, M.K.; Grammenou-Savvoglou, S.; van Winden, E.C.; Dimitriou, A.A.; Demopoulos, C.A.; Tselepis, A.D.; Kotsifaki, E.E.
    Implication of lipoprotein associated phospholipase A2 activity on oxidized low density lipoprotein uptake by macrophages (2010), J. Lipid Res., 51, 2191-2201.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.4

Inhibitors

Inhibitors Comment Organism Structure
4-[2-aminoethyl]benzensulfonyl fluoride pefabloc, inhibition of activity at 1 mM Mus musculus
additional information during low density lipoprotein oxidation with 0.005 mM CuSO4, the remaining Lp-PLA2 activity after 6h is approximately 40-50% while after 24h only 10-15% of the initial activity is preserved Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 Western blot analysis Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 female BALB/c mice
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by gel filtration and on anion exchange column Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
peritoneal macrophage
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-palmitoyl-2-arachidonoyl-phosphatidylcholine + H2O
-
 Mus musculus ?
-
 ?
l-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
-
 Mus musculus ?
-
 ?
oxidized phosphatidylcholine + H2O great amount of oxidized phosphatidylcholine of oxidized LDL is hydrolyzed at the sn-2 position Mus musculus lysophosphatidylcholine + fatty acid
-
 ?

Synonyms

Synonyms Comment Organism
lipoprotein associated phospholipase A2
-
 Mus musculus
Lp-PLA2
-
 Mus musculus

General Information

General Information Comment Organism
malfunction progressive inactivation of Lp-PLA2 during low density lipoprotein oxidation leads to an increased uptake of oxidized low density lipoprotein by macrophages, which can be primarily attributed to the increased uptake of the oxidized phospholipids enriched lipid moiety of oxidized low density lipoprotein. Inhibition of Lp-PLA2 activity has no effect on the uptake of ApoB-liposomes conjugates with ApoB isolated from moderately oxidized low density lipoprotein, moderately oxidized low density lipoprotein with inhibited LpPLA2 activity, heavily oxidized low density lipoprotein and heavily oxidized low density lipoprotein with inhibited LpPLA2 activity Mus musculus
physiological function Lp-PLA2 activity reduces the uptake of oxidized low density lipoprotein by peritoneal macrophages. Lp-PLA2 does not affect the uptake of oxidized low density lipoprotein via the ApoB moiety. Lp-PLA2 reduces the uptake of oxidized low density lipoprotein via the lipid moiety Mus musculus