Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bromoenol lactone | specific iPLA2 inhibitor, which completely suppresses fMLP-induced generation of superoxide. Completely blocks superoxide generation in neutrophils from healthy and diabetic subjects. Exogeneous arachidonic acid rescues neutrophil superoxide inhibition by partly restoring NADPH oxidase activity, resulting in superoxide generation | Homo sapiens | |
pyrrolidine-1 | specific cPLA2 inhibitor, does not inhibit the superoxide generation in healthy neutrophils | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | iPLA2 | Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
x * 85000, iPLA2, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neutrophil | iPLA2 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonyl thio-PC + H2O | - |
Homo sapiens | arachidonic acid + (7R)-4-hydroxy-N,N,N-trimethyl-7-sulfanyl-3,5,9-trioxa-4-phosphapentacosan-1-aminium 4-oxide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 85000, iPLA2, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Ca2+-independent phospholipase A2 | - |
Homo sapiens |
cPLA2 | - |
Homo sapiens |
iPLA2 | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | transfection with iPLA2 siRNA results in ca. 50% reduction in mRNA levels of iPLA2 | down |
Homo sapiens | iPLA2 mRNA expression is increased in neutrophils from people with diabetes and mediates superoxide generation | up |
General Information | Comment | Organism |
---|---|---|
malfunction | small interfering RNA knockdown of iPLA2 inhibits superoxide generation by neutrophils | Homo sapiens |
physiological function | role of iPLA2 in enhanced superoxide generation in neutrophils from people with diabetes mellitus, iPLA2 presents an alternate pathway independent of protein kinase C and phosphatidic acid phosphohydrolase-1 hydrolase signaling. The iPLA2 does not regulate cell apoptosis or cell death in neutrophils, iPLA2 regulates superoxide generation independently of p47phox or pleckstrin phosphorylation. The iPLA2-derived arachidonic acid activates NAPDH oxidase to generate superoxide anion | Homo sapiens |