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Literature summary for 3.1.1.4 extracted from

  • Machado Braga, M.D.; Costa Martins, A.M.; Alves, C.D.; Beserra de Menezes, D.; Martins, R.D.; Ferreira Barbosa, P.S.; Oliveira, I.M.; Toyama, M.H.; Toyama, D.O.; dos Santos Diz Filho, E.B.; Ramos Fagundes, F.H.; Fonteles, M.C.; Azul Monteiro, H.S.
    Purification and renal effects of phospholipase A2 isolated from Bothrops insularis venom (2008), Toxicon, 51, 181-190.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bothrops insularis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Bothrops insularis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13984
-
x * 13984, mass spectrometry, x * 14000, SDS-PAGE Bothrops insularis
14000
-
x * 13984, mass spectrometry, x * 14000, SDS-PAGE Bothrops insularis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bothrops insularis the enzyme shows myonecrotic activity in rat kidneys causing mild to moderate focal acute tubular necrosis in the convoluted proximal tubules, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Bothrops insularis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native PLA2 from venom in several chromatographic steps, including gel filtration and anion exchange chromatography, to over 95% puritiy Bothrops insularis

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Bothrops insularis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitro-3-octanoyloxy-benzoic acid + H2O
-
Bothrops insularis ?
-
?
additional information the enzyme shows myonecrotic activity in rat kidneys causing mild to moderate focal acute tubular necrosis in the convoluted proximal tubules, overview Bothrops insularis ?
-
?

Subunits

Subunits Comment Organism
? x * 13984, mass spectrometry, x * 14000, SDS-PAGE Bothrops insularis

Synonyms

Synonyms Comment Organism
PLA2
-
Bothrops insularis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bothrops insularis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Bothrops insularis

pI Value

Organism Comment pI Value Maximum pI Value
Bothrops insularis isoelectric focusing
-
8.6