Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bothrops insularis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bothrops insularis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13984 | - |
x * 13984, mass spectrometry, x * 14000, SDS-PAGE | Bothrops insularis |
14000 | - |
x * 13984, mass spectrometry, x * 14000, SDS-PAGE | Bothrops insularis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bothrops insularis | the enzyme shows myonecrotic activity in rat kidneys causing mild to moderate focal acute tubular necrosis in the convoluted proximal tubules, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bothrops insularis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native PLA2 from venom in several chromatographic steps, including gel filtration and anion exchange chromatography, to over 95% puritiy | Bothrops insularis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Bothrops insularis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitro-3-octanoyloxy-benzoic acid + H2O | - |
Bothrops insularis | ? | - |
? | |
additional information | the enzyme shows myonecrotic activity in rat kidneys causing mild to moderate focal acute tubular necrosis in the convoluted proximal tubules, overview | Bothrops insularis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 13984, mass spectrometry, x * 14000, SDS-PAGE | Bothrops insularis |
Synonyms | Comment | Organism |
---|---|---|
PLA2 | - |
Bothrops insularis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bothrops insularis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bothrops insularis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bothrops insularis | isoelectric focusing | - |
8.6 |