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Literature summary for 3.1.1.4 extracted from

  • Kim, J.O.; Chakrabarti, B.K.; Guha-Niyogi, A.; Louder, M.K.; Mascola, J.R.; Ganesh, L.; Nabel, G.J.
    Lysis of human immunodeficiency virus type 1 by a specific secreted human phospholipase A2 (2007), J. Virol., 81, 1444-1450.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozymes sPLA2-X in Spodoptery frugiperda Sf9 cells using the baculovirus transfection system, expression of wild-type and D47K mutant isozymes sPLA2-X in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D47E site-directed mutagenesis, inactive mutant Homo sapiens
D47K site-directed mutagenesis, inactive mutant Homo sapiens
H46N site-directed mutagenesis, inactive mutant Homo sapiens
Y50F site-directed mutagenesis, inactive mutant Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secreted PLA2 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens PLA2s affect cellular activation, signal transduction, and possibly innate immunity, the specific secretory isozyme sPLA2-X neutralizes human immunodeficiency virus type 1 through degradation of the viral membrane, the catalytic function is required for antiviral activity, the target cells of infection are unaffected, sPLA2-X potently reduces gene transfer of HIV-1 Env-pseudo-typed lentivirus vectors and inhibits the replication of both CCR5- and CXCR4-tropic HIV-1 in human CD4 T cells. Virions resistant to damage by antibody and complement were sensitive to lysis by sPLA2-X, mechanism, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 secreted human phospholipase A2, i.e. sPLA2-X
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 sPLA2-X antiviral effect, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dithio-heptanoylphosphatidylcholine + H2O detection of reaction product with 5,5'-dithio-bis-(2-nitrobenzoic acid) Homo sapiens ?
-
 ?
additional information PLA2s affect cellular activation, signal transduction, and possibly innate immunity, the specific secretory isozyme sPLA2-X neutralizes human immunodeficiency virus type 1 through degradation of the viral membrane, the catalytic function is required for antiviral activity, the target cells of infection are unaffected, sPLA2-X potently reduces gene transfer of HIV-1 Env-pseudo-typed lentivirus vectors and inhibits the replication of both CCR5- and CXCR4-tropic HIV-1 in human CD4 T cells. Virions resistant to damage by antibody and complement were sensitive to lysis by sPLA2-X, mechanism, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PLA2
-
 Homo sapiens
secreted human phospholipase A2
-
 Homo sapiens
sPLA2-X
-
 Homo sapiens