Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EGTA | - |
Daboia russelii russelii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | absolutely requird for the PLA2 activity of RVV-7 | Daboia russelii russelii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
6500 | 7000 | RVV-7, native PAGE | Daboia russelii russelii |
7201 | 7224 | RVV-7, mass spectrometry | Daboia russelii russelii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Daboia russelii russelii | the PLA2, i.e. RVV-7, is the basic toxin of Russells viper venom, it shows cytotoxic activity with B16F10 melanoma cells, it inhibits trypsin, but not chymotrypsin or bromelain, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daboia russelii russelii | - |
Indian Russell's viper | - |
Purification (Comment) | Organism |
---|---|
native enzyme RVV-7 from venom purified to homogeneity after partial unfolding by 4 M urea followed by ultrafiltration and dialysis | Daboia russelii russelii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate | the active site His48 is conserved in all venom PLA2s | Daboia russelii russelii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Daboia russelii russelii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
RVV-7 cytotoxic activity versus B16F10 melanoma cells, overview | Daboia russelii russelii |
0.121 | - |
purified enzyme | Daboia russelii russelii |
0.347 | - |
crude venom preparation | Daboia russelii russelii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dimyristoyl-sn-glycero-3 phosphocholine + H2O | - |
Daboia russelii russelii | 1-myristoyl-phosphorylcholine + myristic acid | - |
? | |
additional information | the PLA2, i.e. RVV-7, is the basic toxin of Russells viper venom, it shows cytotoxic activity with B16F10 melanoma cells, it inhibits trypsin, but not chymotrypsin or bromelain, overview | Daboia russelii russelii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 7201-7224, mass spetrometry | Daboia russelii russelii |
Synonyms | Comment | Organism |
---|---|---|
RVV-7 | - |
Daboia russelii russelii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Daboia russelii russelii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
3 h, purified RVV-7, loss of 80% of PLA2 activity, the cytotoxic activity is unaffected | Daboia russelii russelii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Daboia russelii russelii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Daboia russelii russelii | isoelectic focusing | - |
9.3 |