Literature summary for 3.1.1.4 extracted from

Bonfim, V.L.; Toyama, M.H.; Novello, J.C.; Hyslop, S.; Oliveira, C.R.; Rodrigues-Simioni, L.; Marangoni, S.
Isolation and enzymatic characterization of a basic phospholipase A2 from Bothrops jararacussu snake venom (2001), J. Protein Chem., 20, 239-245.

Search for more literature for 3.1.1.4

Inhibitors

Inhibitors	Comment	Organism	Structure
crotapotin	-	Bothrops jararacussu
Cu2+	10 mM	Bothrops jararacussu
Zn2+	10 mM	Bothrops jararacussu

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.7	-	4-nitro-3-(octanoyloxy)benzoic acid	pH 8, 37°C	Bothrops jararacussu

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	requirement, optimal concentration: 10 mM	Bothrops jararacussu
Mg2+	10 mM Mg2+ increases the activity slightly in the presence of 1 mM Ca2+	Bothrops jararacussu
Mn2+	10 mM Mn2+ increases the activity slightly in the presence of 1 mM Ca2+	Bothrops jararacussu

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
15000	-	SDS-PAGE	Bothrops jararacussu
30000	-	SDS-PAGE	Bothrops jararacussu

Organism

Organism	UniProt	Comment	Textmining
Bothrops jararacussu	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bothrops jararacussu

Source Tissue

Source Tissue	Comment	Organism	Textmining
venom	-	Bothrops jararacussu	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitro-3-(octanoyloxy)benzoic acid + H2O	synthetic substrate	Bothrops jararacussu	3-hydroxy-4-nitrobenzoate + octanoate	-	?

Subunits

Subunits	Comment	Organism
multimer	enzyme forms multimeric complexes of 30000 Da, 45000 Da and 60000 Da	Bothrops jararacussu

Synonyms

Synonyms	Comment	Organism
BthTX	-	Bothrops jararacussu

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	45	-	Bothrops jararacussu

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.2	-	-	Bothrops jararacussu

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Release 2023.1 (January 2023)