Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.4 extracted from

  • Rehfeldt, W.; Resch, K.; Goppelt-Struebe, M.
    Cytosolic phospholipase A2 from human monocytic cells: characterization of substrate specificity and Ca2+-dependent membrane association (1993), Biochem. J., 293, 255-261.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.4

Activating Compound

Activating Compound Comment Organism Structure
phosphate phosphorylation needed for activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Homo sapiens
EDTA
-
 Homo sapiens
EGTA
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-
plasma membrane
-
 Homo sapiens 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+-dependent PLA2-association with cellular membranes at a concentration of 300-500 nM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 native PAGE Homo sapiens
100000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipids + H2O Homo sapiens eicosanoids ?
-
 ?
phospholipids + H2O Homo sapiens inflammatory diseases ?
-
 ?
phospholipids + H2O Homo sapiens release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
column chromatography, gel electrophoresis, 1206fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte peripheral blood Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0609
-
 substrate: 1-palmitoyl-2-arachidonylphosphatidylcholine Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, with bovine serum albumin, loss of enzyme activity only partially avoided Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-acyl-2-arachidonoyl-sn-glycerophosphoethanolamine + H2O
-
 Homo sapiens 1-acyl-sn-glycerophosphoethanolamine + arachidonic acid
-
 ?
1-palmitoyl-2-arachidonoylphosphatidylcholine + H2O
-
 Homo sapiens 1-palmitoyl-glycerophosphorylcholine + arachidonic acid
-
 ?
phosphatidylcholine + arachidonic acid
-
 Homo sapiens 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine + carboxylic acid
-
 r
phosphatidylcholine + H2O
-
 Homo sapiens 1-acylglycerophosphocholine + fatty acid
-
 ?
phosphatidylcholine + linoleic acid
-
 Homo sapiens lineoyl phosphatidylcholine
-
 ?
phosphatidylethanolamine + arachidonic acid
-
 Homo sapiens arachidonoyl phosphatidylethanolamine
-
 r
phosphatidylethanolamine + linoleic acid
-
 Homo sapiens lineoyl phosphatidylethanolamine + fatty acid
-
 ?
phosphatidylinositol + arachidonic acid
-
 Homo sapiens arachidonoyl phosphatidylinositol + fatty acid
-
 ?
phosphatidylinositol + linoleic acid
-
 Homo sapiens lineoyl phosphatidylinositol + fatty acid
-
 ?
phosphatidylserine + arachidonic acid
-
 Homo sapiens arachidonoyl phosphatidylserine + fatty acid
-
 ?
phosphatidylserine + linoleic acid
-
 Homo sapiens lineoyl phosphatidylserine + fatty acid
-
 ?
phospholipids + H2O eicosanoids Homo sapiens ?
-
 ?
phospholipids + H2O inflammatory diseases Homo sapiens ?
-
 ?
phospholipids + H2O release of arachidonic acid: biosynthesis of leucotrienes, thromboxans and prostaglandins Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Homo sapiens