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Literature summary for 3.1.1.4 extracted from

  • Han, S.K.; Yoon, E.T.; Cho, W.
    Bacterial expression and characterization of human secretory class V phospholipase A2 (1998), Biochem. J., 331, 353-357.
No PubMed abstract available
Search for more literature for 3.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
into pET21a vector, transformation of Escherichia coli strain BL21(DE3) Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
secretory granule
-
 Homo sapiens 30141
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
 heparin-sepharose affinity chromatography, SDS-PAGE after digestion with thrombin, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipids + H2O Homo sapiens eicosanoids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration chromatography Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-4°C, more than 2 weeks without precipitation Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphatidic acid + H2O group V sPLA2 Homo sapiens ?
-
 ?
1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphocholine + H2O group V sPLA2 Homo sapiens 1-hexadecanoyl-glycero-3-phosphocholine + 1-pyrenedecanoate
-
 ?
1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphoethanolamine + H2O group V sPLA2 Homo sapiens 1-hexadecanoyl-glycero-3-phosphoethanolamine + 1-pyrenedecanoate
-
 ?
1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphoglycerol + H2O group V sPLA2 Homo sapiens 1-hexadecanoyl-glycero-3-phosphoglycerol + 1-pyrenedecanoate
-
 ?
1-hexadecanoyl-2-(1-pyrenedecanoyl)-glycero-3-phosphoserine + H2O group V sPLA2 Homo sapiens 1-hexadecanoyl-glycero-3-phosphoserine + 1-pyrenedecanoate
-
 ?
phospholipids + H2O eicosanoids Homo sapiens ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 group V sPLA2 shows a half-life for inactivation of 10 min., group IIa sPLA2 retains 75% of activity after 1 h Homo sapiens