Cloned (Comment) | Organism |
---|---|
DNA and amino acid equence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS, the enzyme is mainly localized in inclusion bodies | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme is a membrane-bound/secreted enzyme in the infective stages of Trypanosoma cruzi | Trypanosoma cruzi | 16020 | - |
additional information | immunohistochemic analysis of subcellular localization | Trypanosoma cruzi | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | H2EIY3 | strains Cvd and RA (lethal) and K98 (non-lethal) | - |
Purification (Comment) | Organism |
---|---|
soluble portion of recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | infective | Trypanosoma cruzi | - |
epimastigote | - |
Trypanosoma cruzi | - |
trypomastigote | - |
Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dipalmitoyl-phosphatidylcholine + H2O | substrate from Glycine max | Trypanosoma cruzi | 2-palmitoyl-sn-glycerophosphocholine + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
PLA1 | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
assay at | Trypanosoma cruzi |
Organism | Comment | Expression |
---|---|---|
Trypanosoma cruzi | increased levels of PLA1 expression in the infective amastigotes and trypomastigotes compared to the non-infective epimastigotes of lethal RA strain | up |
General Information | Comment | Organism |
---|---|---|
physiological function | lysophospholipase activity in live Tryypanosoma cruzi parasites contributes to self protection against toxic compounds generated by phospholipase A1 activity | Trypanosoma cruzi |