Cloned (Comment) | Organism |
---|---|
gene pla, DNA and amino acid sequence determination and analysis, expression and extracellular production of the recombinant enzyme in Streptomyces lividans from expression vector pUC702/pla | Streptomyces albidoflavus |
Protein Variants | Comment | Organism |
---|---|---|
H218A | site-directed mutagenesis, inactive mutant | Streptomyces albidoflavus |
H218R | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Streptomyces albidoflavus |
S11A | site-directed mutagenesis, inactive mutant | Streptomyces albidoflavus |
S11D | site-directed mutagenesis, inactive mutant | Streptomyces albidoflavus |
S11E | site-directed mutagenesis, inactive mutant | Streptomyces albidoflavus |
S11T | site-directed mutagenesis, inactive mutant | Streptomyces albidoflavus |
S11Y | site-directed mutagenesis, inactive mutant | Streptomyces albidoflavus |
S216A | site-directed mutagenesis, inactive mutant | Streptomyces albidoflavus |
S216D | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Streptomyces albidoflavus |
S216E | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Streptomyces albidoflavus |
S216T | site-directed mutagenesis, the mutant shows 80-90% reduced activity compared to the wild-type enzyme | Streptomyces albidoflavus |
S216Y | site-directed mutagenesis, the mutant shows 80-90% reduced activity compared to the wild-type enzyme | Streptomyces albidoflavus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | weak inhibition | Streptomyces albidoflavus | |
Ca2+ | up to 0.1 mM | Streptomyces albidoflavus | |
Fe2+ | - |
Streptomyces albidoflavus | |
Fe3+ | - |
Streptomyces albidoflavus | |
additional information | the enzyme is not inhibited by EDTA and DTT | Streptomyces albidoflavus | |
PMSF | weak inhibition | Streptomyces albidoflavus | |
SDS | - |
Streptomyces albidoflavus | |
Triton X-100 | weak inhibition at up to 0.23% w/v | Streptomyces albidoflavus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.38 | - |
phosphatidic acid | pH 7.2, 50°C, recombinant enzyme | Streptomyces albidoflavus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme possesses a 33-residue N-terminal signal secretion sequence | Streptomyces albidoflavus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates at 10 mM | Streptomyces albidoflavus | |
additional information | metal ion-independent phospholipase A1 | Streptomyces albidoflavus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27199 | - |
1 * 27199, mature protein, seuence calculation, 1 * 28000, recombinant extracellular enzyme, SDS-PAGE | Streptomyces albidoflavus |
28000 | - |
DLS analysis | Streptomyces albidoflavus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces albidoflavus | K0J3J2 | isolated from Japanese soil, gene pla | - |
Streptomyces albidoflavus NA297 | K0J3J2 | isolated from Japanese soil, gene pla | - |
Purification (Comment) | Organism |
---|---|
recombinant extracellular enzyme to homogeneity from the culture supernatant by ammonium sulfate precipitation, hydrophobic interaction chromatography, and anion exchange chromatography | Streptomyces albidoflavus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
588 | 2873 | pH 7.2, 50°C, purified recombinant enzyme | Streptomyces albidoflavus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dimyristoyl-sn-glycero-3-phosphate + H2O | - |
Streptomyces albidoflavus | ? | - |
? | |
1,2-dimyristoyl-sn-glycero-3-phosphate + H2O | - |
Streptomyces albidoflavus NA297 | ? | - |
? | |
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine + H2O | - |
Streptomyces albidoflavus | ? | - |
? | |
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine + H2O | - |
Streptomyces albidoflavus NA297 | ? | - |
? | |
1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O | - |
Streptomyces albidoflavus | ? | - |
? | |
1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O | - |
Streptomyces albidoflavus NA297 | ? | - |
? | |
L-alpha-phosphatidyl-L-serine + H2O | substrate from Glycine max | Streptomyces albidoflavus | 2-acyl-1-glycerophosphoserine + a carboxylate | - |
? | |
L-alpha-phosphatidylcholine + H2O | PLA1 activity for egg yolk lecithin hydrolysis, substrate from egg yolk or Glycine max | Streptomyces albidoflavus | 2-acylglycerophosphocholine + a carboxylate | - |
? | |
L-alpha-phosphatidylglycerol + H2O | - |
Streptomyces albidoflavus | ? | - |
? | |
L-alpha-phosphatidylinositol + H2O | - |
Streptomyces albidoflavus | 2-acyl-sn-glycero-3-phosphoinositol + a carboxylate | - |
? | |
lecithin + H2O | from Glycine max | Streptomyces albidoflavus | ? | - |
? | |
additional information | the substrate specificity is pH-dependent, the enzyme preferably hydrolyzes phosphatidic acid and phosphatidylserine at pH 7.2, it is active with lysophosphatidylcholine, but not with triglyceride and the 4-nitrophenyl ester of fatty acids, substrate specificity, overview. At the reaction equilibrium, the molar ratio of released free fatty acids (sn-1:sn-2) is 63:37 | Streptomyces albidoflavus | ? | - |
? | |
additional information | the substrate specificity is pH-dependent, the enzyme preferably hydrolyzes phosphatidic acid and phosphatidylserine at pH 7.2, it is active with lysophosphatidylcholine, but not with triglyceride and the 4-nitrophenyl ester of fatty acids, substrate specificity, overview. At the reaction equilibrium, the molar ratio of released free fatty acids (sn-1:sn-2) is 63:37 | Streptomyces albidoflavus NA297 | ? | - |
? | |
phosphatidic acid + H2O | - |
Streptomyces albidoflavus | 2-acyl-lysophosphatidic acid + a carboxylate | - |
? | |
phosphatidic acid + H2O | - |
Streptomyces albidoflavus NA297 | 2-acyl-lysophosphatidic acid + a carboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27199, mature protein, seuence calculation, 1 * 28000, recombinant extracellular enzyme, SDS-PAGE | Streptomyces albidoflavus |
Synonyms | Comment | Organism |
---|---|---|
PLA1 | - |
Streptomyces albidoflavus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Streptomyces albidoflavus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | activity range, profile overview | Streptomyces albidoflavus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
native and recombinant enzymes, stable at | Streptomyces albidoflavus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
630 | - |
phosphatidic acid | pH 7.2, 50°C, recombinant enzyme | Streptomyces albidoflavus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Streptomyces albidoflavus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | activity range, profile overview | Streptomyces albidoflavus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.6 | 9 | recombinant enzyme, stable at | Streptomyces albidoflavus |
7.2 | 9 | native enzyme, stable at | Streptomyces albidoflavus |
General Information | Comment | Organism |
---|---|---|
additional information | residue Ser11 is essential for the catalytic function of the enzyme, the active site may include residues Ser216 and His218 | Streptomyces albidoflavus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
265 | - |
phosphatidic acid | pH 7.2, 50°C, recombinant enzyme | Streptomyces albidoflavus |