Cloned (Comment) | Organism |
---|---|
expression of N156A mutant | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
N156A mutant OMPLA is crystallized by hanging drop vapor-diffusion, drops consisting of 0.003 ml of protein solution and 0.002 ml reservoir solution are suspended over a 1 ml reservoir containing 27% 2-methyl-2,4-pentanediol, 0.4-1 mM CaCl2 and 100 mM Bis-Tris buffer, pH 6.0-6.3, the initial protein solution contained 10 mg/ml N156A OMPLA, 10 mM KCl, 1% 1-O-n-octyl-beta-D-glucopyranoside and 0.2 mM Tris-HCl at pH 6.6, crystals diffract to 2.5-3 A | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
outer membrane | - |
Escherichia coli | 19867 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for activity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | Escherichia coli | - |
2-acylglycerophosphocholine + a carboxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
N156A mutant | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | - |
Escherichia coli | 2-acylglycerophosphocholine + a carboxylate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OMPLA | - |
Escherichia coli |
outer membrane phospholipase A | - |
Escherichia coli |