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Literature summary for 3.1.1.31 extracted from

  • Duclert-Savatier, N.; Poggi, L.; Miclet, E.; Lopes, P.; Ouazzani, J.; Chevalier, N.; Nilges, M.; Delarue, M.; Stoven, V.
    Insights into the enzymatic mechanism of 6-phosphogluconolactonase from Trypanosoma brucei using structural data and molecular dynamics simulation (2009), J. Mol. Biol., 388, 1009-1021.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
by the hanging-drop method, 6PGL in complex with two ligands, 6-phosphogluconic acid and citrate, at 2.2 A and 2.0 A resolution, respectively. Structures of the two complexes and of the apo form are almost identical. Both complexes belong to space group P21 and have two molecules in the asymmetric unit, related by a pure translation. PGL in complex with 6-phosphogluconic acid has unit cell parameters of a = 49.66, b = 87, c= 64.91 A and beta = 96.43. PGL in complex with citrate has unit cell parameters of a = 49.46, b = 87.21, c= 64.79 A and beta = 96.53 Trypanosoma brucei

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ has a structural role rather than a catalytic one, lies at the center of a tetrahedron. The side chains of H56 and H97 are at a distance of 2.0 A from the metal ion, while the oxygen atom of the carboxylic side chain of D98 coordinates the zinc ion in a monodentate manner. Water molecule at 2.7 A from the metal ion Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
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Purification (Commentary)

Purification (Comment) Organism
to 95% homogeneity in a single step with an NTA column Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-glucono-1,5-lactone + H2O H165 is the catalytic residue, while R77 and R200 bind to the phosphate group. Ring opening of the lactone involves proton transfer from HE2 of H165 to O1 or O5 of the lactone, via a water molecule Trypanosoma brucei 6-phospho-D-gluconate
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Synonyms

Synonyms Comment Organism
6-phosphogluconolactonase
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Trypanosoma brucei
6PGL
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Trypanosoma brucei