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Literature summary for 3.1.1.3 extracted from

  • Hills, M.J.; Murphy, D.J.
    Characterization of lipases from the lipid bodies and microsomal membranes of erucic acid-free oilseed-rape (Brassica napus) cotyledons (1988), Biochem. J., 249, 687-693.
    View publication on PubMedView publication on EuropePMC
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Localization

Localization Comment Organism GeneOntology No. Textmining
lipid storage body
-
 Brassica napus
-
-
microsome
-
 Brassica napus
-
-

Organism

Organism UniProt Comment Textmining
Brassica napus
-
 cv. Andor
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Brassica napus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
triarachidin + H2O
-
 Brassica napus arachidonic acid + ?
-
 ?
trieicosenoin + H2O
-
 Brassica napus ?
-
 ?
trierucin + H2O
-
 Brassica napus eicosanoic acid + ?
-
 ?
trilaurin + H2O
-
 Brassica napus lauric acid + ?
-
 ?
trilinolein + H2O
-
 Brassica napus linolic acid + ?
-
 ?
trilinolenein + H2O
-
 Brassica napus linolenic acid + ?
-
 ?
trimyristin + H2O
-
 Brassica napus myristic acid + ?
-
 ?
trioleoylglycerol + H2O
-
 Brassica napus oleic acid + ?
-
 ?
tripalmitin + H2O
-
 Brassica napus palmitic acid + ?
-
 ?
tristearin + H2O
-
 Brassica napus stearic acid + ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 microsomal enzyme Brassica napus
9
-
 lipid-body enzyme Brassica napus

pH Range

pH Minimum pH Maximum Comment Organism
6 10 about 30% of maximal activity at pH 6.0 and at pH 10.0, enzyme from microsomal membrane, pH 6.0: about 55% of maximal activity of the enzyme from lipid bodies, pH 10.0: about 50% of maximal activity of the enzyme from lipid bodies Brassica napus