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Literature summary for 3.1.1.3 extracted from

  • Vijayakumar, K.R.; Gowda, L.R.
    Rice (Oryza sativa) lipase: molecular cloning, functional expression and substrate specificity (2013), Protein Expr. Purif., 88, 67-79.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli (DE3)pLysS, RIL(DE3)pLysS, Rosetta(DE3)pLysS and Origami(DE3)pLysS cells (insoluble inclusion bodies) or in Pichia pastoris X-33 cells (secretory protein) Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04533
-
4-nitrophenyl acetate native enzyme, at pH 7.4 and 25°C Oryza sativa
0.1089
-
Triacetin native enzyme, at pH 7.4 and 25°C Oryza sativa
0.1408
-
Triacetin recombinant enzyme, at pH 7.4 and 25°C Oryza sativa
2.766
-
4-nitrophenyl acetate recombinant enzyme, at pH 7.4 and 25°C Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36680
-
x * 36680, calculated from amino acid sequence Oryza sativa
40000
-
x * 40000, SDS-PAGE Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
subspecies Indica, variant IR64
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography Oryza sativa

Renatured (Commentary)

Renatured (Comment) Organism
using 0.05 M phosphate buffer, 2 M urea, 0.5 M oxidized glutathione and 0.001 M reduced glutathione, pH 7.4, at 4°C Oryza sativa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
292.4
-
after 3.41fold purification, with triacetin as substrate, at pH 7.4 and 25°C Oryza sativa
998
-
after 3.41fold purification, with triacetin as substrate, at pH 7.4 and 25°C Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O 36.9% activity compared to rice bran oil Oryza sativa 1-naphthol + acetate
-
?
1-naphthyl butyrate + H2O 18.8% activity compared to rice bran oil Oryza sativa 1-naphthol + butyrate
-
?
4-nitrophenyl acetate + H2O 0.2% activity compared to rice bran oil Oryza sativa 4-nitrophenol + acetate
-
?
coconut oil + H2O 47.9% activity compared to rice bran oil Oryza sativa ?
-
?
olive oil + H2O 95.3% activity compared to rice bran oil Oryza sativa ?
-
?
palm oil + H2O 77.7% activity compared to rice bran oil Oryza sativa ?
-
?
rice bran oil 100% activity Oryza sativa ?
-
?
sunflower oil + H2O 91.7% activity compared to rice bran oil Oryza sativa ?
-
?
triacetin + H2O 120% activity compared to rice bran oil Oryza sativa ?
-
?
tributyrin + H2O 92.6% activity compared to rice bran oil Oryza sativa ?
-
?
trimyristin + H2O 90.1% activity compared to rice bran oil Oryza sativa ?
-
?
tripalmitin + H2O 82.1% activity compared to rice bran oil Oryza sativa ?
-
?
tristearin + H2O 72.9% activity compared to rice bran oil Oryza sativa ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Oryza sativa
? x * 36680, calculated from amino acid sequence Oryza sativa

Synonyms

Synonyms Comment Organism
acylglycerol acylhydrolase
-
Oryza sativa
lipase
-
Oryza sativa
RBL
-
Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
Triacetin native enzyme, at pH 7.4 and 25°C Oryza sativa
0.32
-
Triacetin recombinant enzyme, at pH 7.4 and 25°C Oryza sativa
0.36
-
4-nitrophenyl acetate native enzyme, at pH 7.4 and 25°C Oryza sativa
0.44
-
4-nitrophenyl acetate recombinant enzyme, at pH 7.4 and 25°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Oryza sativa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.4 remains stable Oryza sativa