Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli (DE3)pLysS, RIL(DE3)pLysS, Rosetta(DE3)pLysS and Origami(DE3)pLysS cells (insoluble inclusion bodies) or in Pichia pastoris X-33 cells (secretory protein) | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04533 | - |
4-nitrophenyl acetate | native enzyme, at pH 7.4 and 25°C | Oryza sativa | |
0.1089 | - |
Triacetin | native enzyme, at pH 7.4 and 25°C | Oryza sativa | |
0.1408 | - |
Triacetin | recombinant enzyme, at pH 7.4 and 25°C | Oryza sativa | |
2.766 | - |
4-nitrophenyl acetate | recombinant enzyme, at pH 7.4 and 25°C | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36680 | - |
x * 36680, calculated from amino acid sequence | Oryza sativa |
40000 | - |
x * 40000, SDS-PAGE | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
subspecies Indica, variant IR64 | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography | Oryza sativa |
Renatured (Comment) | Organism |
---|---|
using 0.05 M phosphate buffer, 2 M urea, 0.5 M oxidized glutathione and 0.001 M reduced glutathione, pH 7.4, at 4°C | Oryza sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
292.4 | - |
after 3.41fold purification, with triacetin as substrate, at pH 7.4 and 25°C | Oryza sativa |
998 | - |
after 3.41fold purification, with triacetin as substrate, at pH 7.4 and 25°C | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | 36.9% activity compared to rice bran oil | Oryza sativa | 1-naphthol + acetate | - |
? | |
1-naphthyl butyrate + H2O | 18.8% activity compared to rice bran oil | Oryza sativa | 1-naphthol + butyrate | - |
? | |
4-nitrophenyl acetate + H2O | 0.2% activity compared to rice bran oil | Oryza sativa | 4-nitrophenol + acetate | - |
? | |
coconut oil + H2O | 47.9% activity compared to rice bran oil | Oryza sativa | ? | - |
? | |
olive oil + H2O | 95.3% activity compared to rice bran oil | Oryza sativa | ? | - |
? | |
palm oil + H2O | 77.7% activity compared to rice bran oil | Oryza sativa | ? | - |
? | |
rice bran oil | 100% activity | Oryza sativa | ? | - |
? | |
sunflower oil + H2O | 91.7% activity compared to rice bran oil | Oryza sativa | ? | - |
? | |
triacetin + H2O | 120% activity compared to rice bran oil | Oryza sativa | ? | - |
? | |
tributyrin + H2O | 92.6% activity compared to rice bran oil | Oryza sativa | ? | - |
? | |
trimyristin + H2O | 90.1% activity compared to rice bran oil | Oryza sativa | ? | - |
? | |
tripalmitin + H2O | 82.1% activity compared to rice bran oil | Oryza sativa | ? | - |
? | |
tristearin + H2O | 72.9% activity compared to rice bran oil | Oryza sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Oryza sativa |
? | x * 36680, calculated from amino acid sequence | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
acylglycerol acylhydrolase | - |
Oryza sativa |
lipase | - |
Oryza sativa |
RBL | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Triacetin | native enzyme, at pH 7.4 and 25°C | Oryza sativa | |
0.32 | - |
Triacetin | recombinant enzyme, at pH 7.4 and 25°C | Oryza sativa | |
0.36 | - |
4-nitrophenyl acetate | native enzyme, at pH 7.4 and 25°C | Oryza sativa | |
0.44 | - |
4-nitrophenyl acetate | recombinant enzyme, at pH 7.4 and 25°C | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Oryza sativa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.4 | remains stable | Oryza sativa |