Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain X-33 | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl 4-nitrophenyl phosphate | diethyl 4-nitrophenyl phosphate is only ìnhibitory in the presence of deoxycholic acid sodium salt (2 mM) | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
supernatant, with tributyrin as substrate, at pH 8.0 and 37°C | Sus scrofa |
70 | - |
after 23.33fold purification, with tributyrin as substrate, at pH 8.0 and 37°C | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
olive oil + H2O | - |
Sus scrofa | ? | - |
? | |
tributyrin + H2O | - |
Sus scrofa | ? | - |
? | |
trioctanoyl glycerol + H2O | - |
Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Sus scrofa |
TPL | - |
Sus scrofa |
triacylglycerol acylhydrolase | - |
Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the enzyme is unstable at temperatures over 37°C with less than 40% activity at 40°C, about 25% activity at 45°C, and no activity at 50°C, after 30 min of incubation | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | the enzyme loses 70% of its activity at acid pH, after 5 min of incubation | Sus scrofa |