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Literature summary for 3.1.1.3 extracted from

  • Bou Ali, M.; Karray, A.; Gargouri, Y.; Ben Ali, Y.
    N-terminal domain of turkey pancreatic lipase is active on long chain triacylglycerols and stabilized by colipase (2013), PLoS ONE, 8, e71605.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X-33 Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
diethyl 4-nitrophenyl phosphate diethyl 4-nitrophenyl phosphate is only ìnhibitory in the presence of deoxycholic acid sodium salt (2 mM) Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
supernatant, with tributyrin as substrate, at pH 8.0 and 37°C Sus scrofa
70
-
after 23.33fold purification, with tributyrin as substrate, at pH 8.0 and 37°C Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
olive oil + H2O
-
Sus scrofa ?
-
?
tributyrin + H2O
-
Sus scrofa ?
-
?
trioctanoyl glycerol + H2O
-
Sus scrofa ?
-
?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
lipase
-
Sus scrofa
TPL
-
Sus scrofa
triacylglycerol acylhydrolase
-
Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
the enzyme is unstable at temperatures over 37°C with less than 40% activity at 40°C, about 25% activity at 45°C, and no activity at 50°C, after 30 min of incubation Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 the enzyme loses 70% of its activity at acid pH, after 5 min of incubation Sus scrofa