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Literature summary for 3.1.1.3 extracted from
- Screening for hydrolytic enzymes reveals Ayr1p as a novel triacylglycerol lipase in Saccharomyces cerevisiae (2013), J. Biol. Chem., 288, 36061-36072.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S18A | the mutation completely abolishes lipase activity | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | 2 | 4-nitrophenyl butyrate | at pH 7.4 and 30°C | Saccharomyces cerevisiae |  |
| 3.5 | - |
4-nitrophenyl acetate | at pH 7.4 and 30°C | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| triacylglycerol + H2O | Saccharomyces cerevisiae | - |
diacylglycerol + a carboxylate | - |
? | |
| triacylglycerol + H2O | Saccharomyces cerevisiae TM | - |
diacylglycerol + a carboxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae TM | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Saccharomyces cerevisiae | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Saccharomyces cerevisiae TM | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Saccharomyces cerevisiae | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Saccharomyces cerevisiae TM | 4-nitrophenol + butyrate | - |
? | |
| triacylglycerol + H2O | - |
Saccharomyces cerevisiae | diacylglycerol + a carboxylate | - |
? | |
| triacylglycerol + H2O | - |
Saccharomyces cerevisiae TM | diacylglycerol + a carboxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ayr1p | - |
Saccharomyces cerevisiae |
| TG lipase | - |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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