Literature summary for 3.1.1.3 extracted from

Schlieben, N.H.; Niefind, K.; Schomburg, D.
Expression, purification, and aggregation studies of His-tagged thermoalkalophilic lipase from Bacillus thermocatenulatus (2004), Protein Expr. Purif., 34, 103-110.

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged enzyme is expressed in Escherichia coli	Geobacillus thermocatenulatus

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapour-diffusion method at 20°C. The purified BTL2 lipase shows a strong tendency to aggregate upon concentration, which prevents a reproducible crystallization. Aggregation studies using dynamic light-scattering analysis are performed to improve the purification and concentration of BTL2 lipase. Different chemical classes of additives are tested to manipulate the aggregation behaviour of BTL2 lipase with the aim of obtaining a monodisperse sample to use for crystallization	Geobacillus thermocatenulatus

Crystallization (Comment)

Organism

vapour-diffusion method at 20°C. The purified BTL2 lipase shows a strong tendency to aggregate upon concentration, which prevents a reproducible crystallization. Aggregation studies using dynamic light-scattering analysis are performed to improve the purification and concentration of BTL2 lipase. Different chemical classes of additives are tested to manipulate the aggregation behaviour of BTL2 lipase with the aim of obtaining a monodisperse sample to use for crystallization

Geobacillus thermocatenulatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	x * 40000, SDS-PAGE	Geobacillus thermocatenulatus

Organic Solvent Stability

Organic Solvent	Comment	Organism
2-propanol	for the concentrated lipase, the availability of 5% 2-propanol is sufficient to hold the lipase in monomeric form	Geobacillus thermocatenulatus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus thermocatenulatus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Geobacillus thermocatenulatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl palmitate + H2O	-	Geobacillus thermocatenulatus	4-nitrophenol + palmitate	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000, SDS-PAGE	Geobacillus thermocatenulatus
More	different chemical classes of additives are tested to manipulate the aggregation behaviour of BTL2 lipase with the aim of obtaining a monodisperse sample to use for crystallization. For the process of concentration of BTL2 lipase in monomeric form, 2-propanol and dodecyl dimethylamine-N-oxide are necessary	Geobacillus thermocatenulatus

Synonyms

Synonyms	Comment	Organism
BTL2 lipase	-	Geobacillus thermocatenulatus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	assay at	Geobacillus thermocatenulatus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Geobacillus thermocatenulatus