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Literature summary for 3.1.1.3 extracted from
- Extracellular lipase of Pseudomonas sp. strain ATCC 21808: purification, characterization, crystallization, and preliminary X-ray diffraction data (1991), J. Bacteriol., 173, 4836-4841.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| to establish the best crystallization conditions, the hanging-drop method was used (room temperature). Crystals suitable for X-ray diffraction grow in sitting drops. The lipase crystallizes with different salts and ethylene glycol polymers in the presence of n-octyl-beta-D-glucopyranoside and one alkyloligooxyethylene compound in the range from C5E2 to C8E4. The crystals diffract to a resolution of about 0.25 nm. They belong to space group C2 with lattice constants of a = 9.27 nm, b = 4.74 nm, c = 8.65 nm, and beta = 122.3°, indicating a cell content of one molecule per asymmetric unit of the crystal | Burkholderia cepacia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition with PMSF | Burkholderia cepacia | |
| paraoxon | inhibition of the lipase activities toward emulsified triolein and dissolved p-nitrophenyl acetate by a 1000fold molar excess of paraoxon, 1 h, 99% inhibition | Burkholderia cepacia |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Burkholderia cepacia | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 35000, SDS-PAGE | Burkholderia cepacia |
| 110000 | - |
polyacrylamide gel under nondenaturing conditions | Burkholderia cepacia |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-propanol | 22°C, stable to | Burkholderia cepacia |
| Ethanol | 22°C, crude enzyme is significantly more resistant to higher concentrations of ethanol (above 40%) than to lower concentrations (20%), whereas purified lipase is inactivated by concentrations of above 40% | Burkholderia cepacia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | P22088 | i.e. Pseudomonas cepacia | - |
| Burkholderia cepacia ATCC 21808 | P22088 | i.e. Pseudomonas cepacia | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chromatography on Q-Sepharose in the presence of n-octyl-beta-D-glucopyranoside, Ca2+ precipitation of fatty acids, and octyl-Sepharose chromatography | Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificity for saturated fatty acids from C6 to C12 and unsaturated long-chain fatty acids. Monoglycerides are hydrolyzed very slowly | Burkholderia cepacia | ? | - |
? | |
| additional information | substrate specificity for saturated fatty acids from C6 to C12 and unsaturated long-chain fatty acids. Monoglycerides are hydrolyzed very slowly | Burkholderia cepacia ATCC 21808 | ? | - |
? | |
| triolein + H2O | - |
Burkholderia cepacia | diolein + oleate | - |
? | |
| triolein + H2O | - |
Burkholderia cepacia ATCC 21808 | diolein + oleate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | Burkholderia cepacia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Burkholderia cepacia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Burkholderia cepacia |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Burkholderia cepacia | isoelectric focusing | 4.6 | 4.5 |
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