Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 cells | Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
I56T | the mutant shows 3fold improved thermostability at 50°C compared to the wild type enzyme, the specific activity of the mutant enzyme is enhanced approximately twice in comparison to wild type with preference for long chain triacylglycerols | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 24000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzyme I56T is purified by Ni-NTA column chromatography | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32 | - |
wild type enzyme from culture supernatant, pH 8.0, 37°C | Bacillus sp. (in: Bacteria) |
3170 | - |
mutant enzyme I56T from culture supernatant, pH 8.0, 37°C | Bacillus sp. (in: Bacteria) |
5834 | - |
mutant enzyme I56T after 208fold purification, pH 8.0, 37°C | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | about 45% activity compared to 4-nitrophenyl butyrate | Bacillus sp. (in: Bacteria) | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | best substrate for wild type enzyme | Bacillus sp. (in: Bacteria) | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | about 100% activity compared to 4-nitrophenyl butyrate | Bacillus sp. (in: Bacteria) | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl laurate + H2O | about 80% activity compared to 4-nitrophenyl butyrate | Bacillus sp. (in: Bacteria) | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl myristate + H2O | about 30% activity compared to 4-nitrophenyl butyrate | Bacillus sp. (in: Bacteria) | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl palmitate + H2O | about 30% activity compared to 4-nitrophenyl butyrate | Bacillus sp. (in: Bacteria) | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl stearate + H2O | about 15% activity compared to 4-nitrophenyl butyrate | Bacillus sp. (in: Bacteria) | 4-nitrophenol + stearate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Bacillus sp. (in: Bacteria) |
triacylglycerol acylhydrolase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | - |
wild type enzyme | Bacillus sp. (in: Bacteria) |
38 | - |
mutant enzyme I56T | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the wild type enzyme shows a half-life of 7 min at 50°C, the mutant enzyme I567T shows a half-life of 21 min at 50°C | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | mutant enzyme I56T | Bacillus sp. (in: Bacteria) |
7 | 10 | wild type enzyme | Bacillus sp. (in: Bacteria) |