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Literature summary for 3.1.1.3 extracted from
- Purification and properties of digestive lipases from Chinook salmon (Oncorhynchus tshawytscha) and New Zealand hoki (Macruronus novaezelandiae) (2010), Fish Physiol. Biochem., 36, 1041-1060.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Colipase | 113% activity at 0.01 mM | Macruronus novaezelandiae | |
| additional information | not influenced by colipase and potassium iodide | Oncorhynchus tshawytscha | |
| sodium cholate | absolutely necessary both for lipase stability in the gel and for optimum activity, 5 mM improves the lipase activity approximately 100fold | Oncorhynchus tshawytscha |  |
| taurocholate | absolutely necessary both for lipase stability in the gel and for optimum activity, 10 mM improves the lipase activity approximately 130fold | Oncorhynchus tshawytscha | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 7% residual activity at 10 mM | Macruronus novaezelandiae | |
| Fe2+ | 14% residual activity at 10 mM | Oncorhynchus tshawytscha | |
| Hg2+ | complete inhibition at 1 mM | Macruronus novaezelandiae | |
| Hg2+ | complete inhibition at 1 mM | Oncorhynchus tshawytscha | |
| Mg2+ | 86% residual activity at 1 mM | Macruronus novaezelandiae | |
| Mg2+ | 80% residual activity at 1 mM | Oncorhynchus tshawytscha | |
| NaN3 | 78% residual activity at 10 mM | Macruronus novaezelandiae | |
| NaN3 | 75% residual activity at 1 mM | Oncorhynchus tshawytscha | |
| paraoxon | - |
Macruronus novaezelandiae | |
| paraoxon | - |
Oncorhynchus tshawytscha | |
| phenylmethylsulfonyl fluoride | 54% residual activity at 5 mM | Macruronus novaezelandiae | |
| phenylmethylsulfonyl fluoride | 36% residual activity at 5 mM | Oncorhynchus tshawytscha | |
| Triton X-100 | complete inhibition at 10% (v/v) | Macruronus novaezelandiae | |
| Triton X-100 | complete inhibition at 10% (v/v) | Oncorhynchus tshawytscha | |
| Tween 80 | 20% residual activity at 10% (v/v) | Macruronus novaezelandiae | |
| Tween 80 | 33% residual activity at 10% (v/v) | Oncorhynchus tshawytscha | |
| Zn2+ | 49% residual activity at 10 mM | Macruronus novaezelandiae | |
| Zn2+ | 51% residual activity at 10 mM | Oncorhynchus tshawytscha | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.068 | - |
4-nitrophenyl caprate | pH 8.0, 30°C | Macruronus novaezelandiae | |
| 0.078 | - |
4-nitrophenyl caprate | pH 8.0, 30°C | Oncorhynchus tshawytscha | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | absolutely necessary both for lipase stability in the gel and for optimum activity | Macruronus novaezelandiae | |
| Ca2+ | absolutely necessary both for lipase stability in the gel and for optimum activity, improves the lipase activity approximately 100fold | Oncorhynchus tshawytscha | |
| Mn2+ | 113% activity at 10 mM | Macruronus novaezelandiae | |
| Mn2+ | 143% activity at 10 mM | Oncorhynchus tshawytscha | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, calculated from SDS-PAGE | Macruronus novaezelandiae |
| 54900 | - |
x * 54900, final enzyme form, calculated from SDS-PAGE | Oncorhynchus tshawytscha |
| 79600 | - |
x * 79600, uncleaved enzyme form, calculated from SDS-PAGE | Oncorhynchus tshawytscha |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | complete inhibition at 50% (v/v) | Oncorhynchus tshawytscha |
| Acetone | complete inhibition at 50% (v/v) | Macruronus novaezelandiae |
| hexane | 82% residual activity at 100% (v/v) | Oncorhynchus tshawytscha |
| hexane | 90% residual activity at 100% (v/v) | Macruronus novaezelandiae |
| isooctane | 58% residual activity at 100% (v/v) | Oncorhynchus tshawytscha |
| isooctane | 71% residual activity at 100% (v/v) | Macruronus novaezelandiae |
| isopropanol | 23% residual activity at 50% (v/v) | Oncorhynchus tshawytscha |
| isopropanol | 36% residual activity at 50% (v/v) | Macruronus novaezelandiae |
| Methanol | complete inhibition at 50% (v/v) | Oncorhynchus tshawytscha |
| Methanol | complete inhibition at 50% (v/v) | Macruronus novaezelandiae |
| n-heptane | 103% activity at 100% (v/v) | Macruronus novaezelandiae |
| n-heptane | 95% residual activity at 100% (v/v) | Oncorhynchus tshawytscha |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Macruronus novaezelandiae | - |
New Zealand hoki | - |
| Oncorhynchus tshawytscha | - |
Chinook salmon | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| fractional precipitation with polyethylene glycol 1000, cholate-Affi-Gel 102 affinity chromatography, and Sephacryl S-300 gel filtration | Oncorhynchus tshawytscha |
| fractional precipitation with polyethylene glycol 1000, cholate-Affi-Gel 102 affinity chromatography, and Sephacryl S-300 gel filtration | Macruronus novaezelandiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pyloric cecum | - |
Oncorhynchus tshawytscha | - |
| pyloric cecum | - |
Macruronus novaezelandiae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.36 | - |
crude extract, pH 8.0, 30°C | Oncorhynchus tshawytscha |
| 4.8 | - |
after 14fold purification pH 8.0, 30°C | Oncorhynchus tshawytscha |
| 4.8 | - |
after 14fold purification pH 8.0, 30°C | Macruronus novaezelandiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl butyrate + H2O | - |
Oncorhynchus tshawytscha | 4-methylumbelliferone + butyrate | - |
? | |
| 4-methylumbelliferyl butyrate + H2O | - |
Macruronus novaezelandiae | 4-methylumbelliferone + butyrate | - |
? | |
| 4-methylumbelliferyl oleate + H2O | - |
Oncorhynchus tshawytscha | 4-methylumbelliferone + oleate | - |
? | |
| 4-methylumbelliferyl oleate + H2O | - |
Macruronus novaezelandiae | 4-methylumbelliferone + oleate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Oncorhynchus tshawytscha | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Macruronus novaezelandiae | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl caprate + H2O | best substrate | Oncorhynchus tshawytscha | 4-nitrophenol + caprate | - |
? | |
| 4-nitrophenyl caprate + H2O | best substrate | Macruronus novaezelandiae | 4-nitrophenol + caprate | - |
? | |
| 4-nitrophenyl caprate + H2O | highest lipase activity is obtained with 0.25 mM of the substrate | Oncorhynchus tshawytscha | 4-nitrophenol + caprate | - |
? | |
| 4-nitrophenyl caprate + H2O | highest lipase activity is obtained with 0.25 mM of the substrate | Macruronus novaezelandiae | 4-nitrophenol + caprate | - |
? | |
| 4-nitrophenyl palmitate + H2O | highest lipase activity is obtained with 0.25 mM of the substrate | Oncorhynchus tshawytscha | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | highest lipase activity is obtained with 0.25 mM of the substrate | Macruronus novaezelandiae | 4-nitrophenol + palmitate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, calculated from SDS-PAGE | Macruronus novaezelandiae |
| ? | x * 54900, final enzyme form, calculated from SDS-PAGE | Oncorhynchus tshawytscha |
| ? | x * 79600, uncleaved enzyme form, calculated from SDS-PAGE | Oncorhynchus tshawytscha |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Oncorhynchus tshawytscha |
| lipase | - |
Macruronus novaezelandiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Oncorhynchus tshawytscha |
| 35 | - |
- |
Macruronus novaezelandiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Oncorhynchus tshawytscha |
| 8 | 8.5 | - |
Macruronus novaezelandiae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
paraoxon | pH 8.0, 30°C | Oncorhynchus tshawytscha | |
| 0.00517 | - |
paraoxon | pH 8.0, 30°C | Macruronus novaezelandiae | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Oncorhynchus tshawytscha | isoelectric focusing | - |
5.7 |
| Macruronus novaezelandiae | isoelectric focusing | - |
5.8 |
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