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Literature summary for 3.1.1.3 extracted from
- Purification and characterization of an intracellular lipase from pleopods of whiteleg shrimp (Litopenaeus vannamei) (2011), Comp. Biochem. Physiol. B, 158, 99-105.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyl 4-nitrophenyl phosphate | 10% residual activity at 10 mM | Penaeus vannamei |  |
| tetrahydrolipstatin | complete inhibition at 1 mM | Penaeus vannamei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 41.03 | - |
4-Methylumbelliferyl butyrate | apparent value, in 2.5 mM Tris-HCl, at pH 8.0 at 30°C | Penaeus vannamei | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Penaeus vannamei | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the lipase is not dependent on Ca2+ for activation or stability | Penaeus vannamei |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63360 | - |
1 * 95260 + 1 * 63360, calculated from SDS-PAGE | Penaeus vannamei |
| 95260 | - |
1 * 95260 + 1 * 63360, calculated from SDS-PAGE | Penaeus vannamei |
| 196000 | - |
gel filtration | Penaeus vannamei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penaeus vannamei | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | the enzyme lacks glycosylation | Penaeus vannamei |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phenyl-Sepharose column chromatography, gel filtration | Penaeus vannamei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| digestive gland | - |
Penaeus vannamei | - |
| pleopod | - |
Penaeus vannamei | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.83 | - |
crude extract, using triolein as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
| 27.2 | - |
purified enzyme, using 2-naphthyl caprylate as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
| 71.5 | - |
purified enzyme, using 2-naphthyl acetate as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
| 85.1 | - |
purified enzyme, using 1-naphthyl acetate as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
| 213.6 | - |
purified enzyme, using tripalmitin as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
| 215 | - |
purified enzyme, using tributyrin as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
| 416.5 | - |
purified enzyme, using tristearin as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
| 922.2 | - |
after 58.25fold purification, using triolein as substrate, at pH 8.0 at 30°C | Penaeus vannamei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl acetate + H2O | - |
Penaeus vannamei | 1-naphthol + acetate | - |
? | |
| 2-naphthyl acetate + H2O | - |
Penaeus vannamei | 2-naphthol + acetate | - |
? | |
| 2-naphthyl caprylate + H2O | - |
Penaeus vannamei | 2-naphthol + caprylate | - |
? | |
| 4-methylumbelliferyl butyrate + H2O | - |
Penaeus vannamei | 4-methylumbelliferone + butyrate | - |
? | |
| triolein + H2O | - |
Penaeus vannamei | ? | - |
? | |
| tripalmitin + H2O | - |
Penaeus vannamei | ? | - |
? | |
| tristearin + H2O | - |
Penaeus vannamei | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 95260 + 1 * 63360, calculated from SDS-PAGE | Penaeus vannamei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Penaeus vannamei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | - |
Penaeus vannamei |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | at 20°C and at 50°C, about 50% of the activity is lost and at 60°C, activity falls abruptly by denaturation | Penaeus vannamei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 200.2 | - |
4-Methylumbelliferyl butyrate | apparent value, in 2.5 mM Tris-HCl, at pH 8.0 at 30°C | Penaeus vannamei | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | - |
Penaeus vannamei |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Penaeus vannamei | isoelectric focusing | - |
5 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 1 | - |
in 2.5 mM Tris-HCl, at pH 8.0 at 30°C | Penaeus vannamei | diethyl 4-nitrophenyl phosphate | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the lipase has a potential role in hydrolysis of triacylglycerides stored as fat body | Penaeus vannamei |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.88 | - |
4-Methylumbelliferyl butyrate | apparent value, in 2.5 mM Tris-HCl, at pH 8.0 at 30°C | Penaeus vannamei | |
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