Organism | UniProt | Comment | Textmining |
---|---|---|---|
Diutina rugosa | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, Al2O3-immobilized lipase in 50 mM phosphate buffer (pH 8.0), the immobilized lipase retains 90% of its catalytic activity | Diutina rugosa |
4°C, free enzyme in 50 mM phosphate buffer (pH 8.0), over a period of 4 months, the activity o the free enzyme gradually decreases to 50% | Diutina rugosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme uses olive oil as substrate | Diutina rugosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Diutina rugosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
free enzyme | Diutina rugosa |
40 | - |
Al2O3-immobilized enzyme | Diutina rugosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 70 | the free enzyme shows full activity after 1 h at 5°C, about 80% activity at 25-35°C, about 65% activity at 40-45°C, about 50% activity at 50°C and is completely inactive after 1 h at 60°C. The immobilized lipase holds approximately 30% activity at 55°C, while the free lipase remains less than 10% | Diutina rugosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
free enzyme | Diutina rugosa |
8.5 | - |
Al2O3-immobilized enzyme | Diutina rugosa |