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Literature summary for 3.1.1.3 extracted from

  • Zhao, L.; Xu, J.; Zhao, J.; Pan, J.; Wang, Z.
    Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010 (2008), Process Biochem., 43, 626-633.
No PubMed abstract available
Search for more literature for 3.1.1.3

Activating Compound

Activating Compound Comment Organism Structure
Gum arabic 142% relative activity at 1% (w/v) Serratia marcescens
Pg350me 117% relative activity at 1% (w/v) Serratia marcescens
polyvinyl alcohol 118% relative activity at 1% (w/v) Serratia marcescens

Inhibitors

Inhibitors Comment Organism Structure
acetone 98.3% inhibition at 50% (v/v) Serratia marcescens
Ca2+ 95.0% residual activity at 10 mM Serratia marcescens
Co2+ 46.0% residual activity at 10 mM Serratia marcescens
Cu2+ 37.0% residual activity at 10 mM Serratia marcescens
EDTA 16.0% residual activity at 10 mM Serratia marcescens
ethanol 98.8% inhibition at 50% (v/v) Serratia marcescens
Fe2+ 14.0% residual activity at 10 mM Serratia marcescens
Fe3+ 12% residual activity at 10 mM Serratia marcescens
Isopropanol 98.9% inhibition at 50% (v/v) Serratia marcescens
K+ 89.0% residual activity at 10 mM Serratia marcescens
methanol 99% inhibition at 50% (v/v) Serratia marcescens
Mg2+ 88.0% residual activity at 10 mM Serratia marcescens
Mn2+ 60.0% residual activity at 10 mM Serratia marcescens
Na+ 90.0% residual activity at 10 mM Serratia marcescens
Ni2+ 40.0% residual activity at 10 mM Serratia marcescens
Pg250de 78% residual activity at 1% (v/v) Serratia marcescens
Pg400de 96% residual activity at 1% (v/v) Serratia marcescens
Triton X-100 inhibits the activity seriously Serratia marcescens
Triton X-45 inhibits the activity seriously Serratia marcescens
Tween 80 72% residual activity at 1% (v/v) Serratia marcescens
Zn2+ 16.2% residual activity at 10 mM Serratia marcescens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not activated by Ca2+ Serratia marcescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 SDS-PAGE Serratia marcescens
140000
-
 native lipase, gel filtration Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
-
-
Serratia marcescens ECU1010
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Toyopearl 650 M column chromatography, Sephadex G150 gel filtration, and phenyl-Toyopearl 650 M column chromatography Serratia marcescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90
-
 crude enzyme, using 4-nitrophenyl butyrate as substrate, in 100 mM potassium phosphate buffer (pH 7.0), at 30°C Serratia marcescens
497
-
 after 5.5fold purification, using 4-nitrophenyl butyrate as substrate, in 100 mM potassium phosphate buffer (pH 7.0), at 30°C Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-one acetate + H2O 50% conversion Serratia marcescens ?
-
 ?
4-nitrophenyl acetate + H2O
-
 Serratia marcescens 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Serratia marcescens ECU1010 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Serratia marcescens 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Serratia marcescens ECU1010 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl decanoate + H2O
-
 Serratia marcescens 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl hexanoate + H2O
-
 Serratia marcescens 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl hexanoate + H2O
-
 Serratia marcescens ECU1010 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl laurate + H2O
-
 Serratia marcescens 4-nitrophenol + laurate
-
 ?
4-nitrophenyl myristate + H2O the enzyme shows maximum activity on 4-nitrophenyl myristate Serratia marcescens 4-nitrophenol + myristate
-
 ?
4-nitrophenyl palmitate + H2O
-
 Serratia marcescens 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl propionate + H2O
-
 Serratia marcescens 4-nitrophenol + propionate
-
 ?
4-nitrophenyl propionate + H2O
-
 Serratia marcescens ECU1010 4-nitrophenol + propionate
-
 ?
4-phenyl-[1,3]-dioxolan-2-one + H2O 9.6% conversion Serratia marcescens ?
-
 ?
glycidyl butyrate + H2O 49% conversion Serratia marcescens ?
-
 ?
methyl acetate + H2O 25% conversion Serratia marcescens ?
-
 ?
methyl mandelate + H2O 33% conversion Serratia marcescens ?
-
 ?
additional information does not hydolyze propylene carbonate Serratia marcescens ?
-
 ?
additional information does not hydolyze propylene carbonate Serratia marcescens ECU1010 ?
-
 ?
naproxen methyl ester + H2O 41% conversion Serratia marcescens ?
-
 ?
trans-3-(4'-methoxyphenyl) glycidic acid methyl ester + H2O 60% conversion Serratia marcescens ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 65000, native lipase, gel filtration Serratia marcescens

Synonyms

Synonyms Comment Organism
lipase
-
 Serratia marcescens
triacylglycerol hydrolase
-
 Serratia marcescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 retains 71% activity after incubation at 50°C for 1 h Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Serratia marcescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9
-
 Serratia marcescens

pI Value

Organism Comment pI Value Maximum pI Value
Serratia marcescens isoelectric focusing
-
 4.2