Activating Compound | Comment | Organism | Structure |
---|---|---|---|
olive oil | best inducer for the production of lipase | Burkholderia cepacia |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain ER2566 | Burkholderia cepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
4-nitrophenyl palmitate | at pH 7 and 37°C | Burkholderia cepacia | |
16.6 | - |
tributyrin | at pH 7 and 37°C | Burkholderia cepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Burkholderia cepacia |
Organic Solvent | Comment | Organism |
---|---|---|
Triton X-100 | stable in the presence of the detergent Triton X-100 | Burkholderia cepacia |
Tween | stable in the presence of the detergent Tween 20 | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | A9QXC9 | strain ATCC 25609, formerly called Pseudomonas cepacia | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Macro-Prep methyl column chromatography | Burkholderia cepacia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
230 | - |
crude extract, at pH 7 and 37°C | Burkholderia cepacia |
8830 | - |
after 38fold purification, at pH 7 and 37°C | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl palmitate + H2O | - |
Burkholderia cepacia | 4-nitrophenol + palmitate | - |
? | |
tributyrin + H2O | - |
Burkholderia cepacia | dibutyrin + butyrate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Burkholderia cepacia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Burkholderia cepacia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | half-life is 54 and 46 min at 50 and 60°C respectively | Burkholderia cepacia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Burkholderia cepacia |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | stable up to 12 h at pH 9 and 10, loses up to 50% activity after 12 h at pH 8 | Burkholderia cepacia |