Literature summary for 3.1.1.3 extracted from

Hilterhaus, L.; Minow, B.; Mueller, J.; Berheide, M.; Quitmann, H.; Katzer, M.; Thum, O.; Antranikian, G.; Zeng, A.P.; Liese, A.
Practical application of different enzymes immobilized on sepabeads (2008), Bioprocess Biosyst. Eng., 31, 163-171.

Application

Application	Comment	Organism
biotechnology	biocatalytic surfactant production, for example the synthesis of myristyl myristate.	Moesziomyces antarcticus

General Stability

General Stability	Organism
The stability of Sepabeads EC-EP-CALB is investigated within the solvent-free system polyglycerol+lauric acid. The limiting factor of this system is its high viscosity and the different polarities of the starting materials. This causes a two phase system, whereby the detergent form formed as a product accounts for some leaching of the biocatalyst. These are challenges for the optimized use of the prepared biocatalyst. Using it in a repetitive batch mode gives information about the stability in the esterification reaction. The initial reaction rate is found to be 500 U/g and decreases within eight batches to 130 U/g.	Moesziomyces antarcticus

Organism

The stability of Sepabeads EC-EP-CALB is investigated within the solvent-free system polyglycerol+lauric acid. The limiting factor of this system is its high viscosity and the different polarities of the starting materials. This causes a two phase system, whereby the detergent form formed as a product accounts for some leaching of the biocatalyst. These are challenges for the optimized use of the prepared biocatalyst. Using it in a repetitive batch mode gives information about the stability in the esterification reaction. The initial reaction rate is found to be 500 U/g and decreases within eight batches to 130 U/g.

Moesziomyces antarcticus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	-	Moesziomyces antarcticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Moesziomyces antarcticus	The conversion of the lauric acid is at 80% after 4 h whereas the residual amount of polyglycerol is at 50% at this time. The conversion of polyglycerol remains lower than the conversion of lauric acid, because of the multiple hydroxy functions within the mixture of different glycerol oligomers. The equilibrium of the reaction can be shifted to total conversion by removal of water applying vacuum to the stirred tank reactor.	?	-	?
additional information	Moesziomyces antarcticus	The synthesis of myristyl myristate by Sepabeads EC-EP/CALB is an example for a biocatalytic surfactant production.	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Moesziomyces antarcticus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	Immobilization of CALB on Sepabeads EC-EP support	Moesziomyces antarcticus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	Comparing the course of this reaction for myristyl myristate, to that catalyzed by Novozym 435, the biocatalyst Sepabeads EC-EP-CALB shows similar activity.	Moesziomyces antarcticus
additional information	-	The CALB-Sepabeads EC-EP biocatalyst with an enzyme loading of 100 mg/g exhibits high activity towards the direct esterification of polyglycerol and lauric acid.	Moesziomyces antarcticus
additional information	-	Using the immobilized lipase B, the synthesis of the bulk chemicals myristyl myristate and polyglycerol laurate can be carried out with an initial reaction rate of 0.40 and 1.24 kU/g. The corresponding half-life time is determined to be 12.9 hours.	Moesziomyces antarcticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	The conversion of the lauric acid is at 80% after 4 h whereas the residual amount of polyglycerol is at 50% at this time. The conversion of polyglycerol remains lower than the conversion of lauric acid, because of the multiple hydroxy functions within the mixture of different glycerol oligomers. The equilibrium of the reaction can be shifted to total conversion by removal of water applying vacuum to the stirred tank reactor.	Moesziomyces antarcticus	?	-	?
additional information	The synthesis of myristyl myristate by Sepabeads EC-EP/CALB is an example for a biocatalytic surfactant production.	Moesziomyces antarcticus	?	-	?

Synonyms

Synonyms	Comment	Organism
CALB	-	Moesziomyces antarcticus
lipase B	-	Moesziomyces antarcticus