Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sodium deoxycholate | 23% increase in activity at 0.5% (w/v) | Bacillus cereus | |
sodium taurocholate | 20% increase in activity at 0.5% (w/v) | Bacillus cereus |
General Stability | Organism |
---|---|
the enzyme remains stable in the presence of trypsin | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzalkonium chloride | little inhibitory effect | Bacillus cereus | |
Brij 35 | little inhibitory effect | Bacillus cereus | |
cetyltrimethylammonium bromide | - |
Bacillus cereus | |
cholic acid | 90% residual activity at 0.5% (w/v) | Bacillus cereus | |
Co2+ | 38.83% residual activity at 5 mM | Bacillus cereus | |
Fe3+ | complete inhibition at 5 mM | Bacillus cereus | |
Mn2+ | complete inhibition at 5 mM | Bacillus cereus | |
Polyethylene glycol | little inhibitory effect | Bacillus cereus | |
Sn2+ | complete inhibition at 5 mM | Bacillus cereus | |
Span 40 | little inhibitory effect | Bacillus cereus | |
Triton X-100 | - |
Bacillus cereus | |
Tween 20 | little inhibitory effect | Bacillus cereus | |
Tween 80 | little inhibitory effect | Bacillus cereus | |
Zn2+ | 72.41% residual activity at 5 mM | Bacillus cereus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 104% increase in activity in the presence of 1 mM Ca2+ | Bacillus cereus | |
Co2+ | 112.56% increase in activity in the presence of 0.1 mM Co2+, the stimulatory effect is not observed at higher concentrations | Bacillus cereus | |
Cu2+ | 83.03% increase in activity in the presence of 1 mM Cu2+ | Bacillus cereus | |
Mg2+ | 46.66% increase in activity in the presence of 0.1 mM Mg2+, the stimulatory effect is not observed at higher concentrations | Bacillus cereus | |
Zn2+ | 41.5% increase in activity in the presence of 1 mM Zn2+, the stimulatory effect is not observed at higher concentrations | Bacillus cereus |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | commercial detergents enhance the enzyme activity, the enzyme retains 60% or more activity in presence of commercial detergents | Bacillus cereus |
SDS | SDS has little stimulatory effect | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus cereus C(7) | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme is stable in the presence of oxidizing agents like 1% (v/v) hydrogen peroxide and 1% (w/v) sodium hypochlorite | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
olive oil + H2O | - |
Bacillus cereus | ? | - |
? | |
olive oil + H2O | - |
Bacillus cereus C(7) | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | lipase activity increases progressively with increase in temperature and maximum activity is found to be at 60°C, then the activity decreases with further increase in temperature | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 9 | at 60°C, using Tris-HCl buffer | Bacillus cereus |
9 | 10 | at 60°C, using glycine-NaOH buffer | Bacillus cereus |