Cloned (Comment) | Organism |
---|---|
gene lip7, DNA and amino acid sequence determination and anaylsis, expression of His6-tagged LipY7p in Pichia pastoris strain KM71, expression pattern, overview | Yarrowia lipolytica |
gene lip8, DNA and amino acid sequence determination and anaylsis, expression of His6-tagged LipY8p in Pichia pastoris strain KM71, expression pattern, overview | Yarrowia lipolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
x * 41000, recombinant LipY7p, SDS-PAGE | Yarrowia lipolytica |
41000 | - |
x * 41000, recombinant LipY8p, SDS-PAGE | Yarrowia lipolytica |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | at 50%, 3 h, room temperature, purified recombinant LipY7p, over 60% remaining activity | Yarrowia lipolytica |
DMSO | at 50%, 3 h, room temperature, purified recombinant LipY8p, over 60% remaining activity | Yarrowia lipolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yarrowia lipolytica | Q872L3 | LIPY8 | - |
Yarrowia lipolytica | Q872L4 | LipY7p | - |
Yarrowia lipolytica AS 2.1216 | Q872L3 | LIPY8 | - |
Yarrowia lipolytica AS 2.1216 | Q872L4 | LipY7p | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant LipY7p expressed in Pichia pastoris is slightly glycosylated | Yarrowia lipolytica |
glycoprotein | the recombinant LipY8p expressed in Pichia pastoris is slightly glycosylated | Yarrowia lipolytica |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged LipY7p from Pichia pastoris strain KM71 by nickel affinity chromatography | Yarrowia lipolytica |
recombinant His6-tagged LipY8p from Pichia pastoris strain KM71 by nickel affinity chromatography | Yarrowia lipolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
57.8 | - |
purified recombinant LipY8p | Yarrowia lipolytica |
62.6 | - |
purified recombinant LipY7p | Yarrowia lipolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl laurate + H2O | - |
Yarrowia lipolytica | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Yarrowia lipolytica AS 2.1216 | 4-nitrophenol + laurate | - |
? | |
additional information | substrate specificity of LipY8p, overview | Yarrowia lipolytica | ? | - |
? | |
additional information | substrate specificity of LipY8p, overview | Yarrowia lipolytica AS 2.1216 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41000, recombinant LipY7p, SDS-PAGE | Yarrowia lipolytica |
? | x * 41000, recombinant LipY8p, SDS-PAGE | Yarrowia lipolytica |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Yarrowia lipolytica |
LipY7p | - |
Yarrowia lipolytica |
LipY8p | - |
Yarrowia lipolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Yarrowia lipolytica |
45 | - |
- |
Yarrowia lipolytica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
3 h, purified recombinant LipY7p, pH 7.0, 85% remaining activity | Yarrowia lipolytica |
65 | - |
3 h, purified recombinant LipY8p, pH 7.0, 85% remaining activity | Yarrowia lipolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Yarrowia lipolytica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | 3 h, room temperature, recombinant LipY7p, 75% remaining activity | Yarrowia lipolytica |
5 | 10 | 3 h, room temperature, recombinant LipY8p, 75% remaining activity | Yarrowia lipolytica |