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Literature summary for 3.1.1.3 extracted from

  • Soliman, N.A.; Knoll, M.; Abdel-Fattah, Y.R.; Schmid, R.D.; Lange, S.
    Molecular cloning and characterization of thermostable esterase and lipase from Geobacillus thermoleovorans YN isolated from desert soil in Egypt (2007), Process Biochem., 42, 1090-1100.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Geobacillus thermoleovorans
DTT
-
Geobacillus thermoleovorans

Cloned(Commentary)

Cloned (Comment) Organism
gene lipA, DNA and amino acid sequence determination and anaylsis, phylogenetic analysis, expression of His6-tagged enzyme in Escherichia coli strain DH5alpha under control of the temperature-inducible lambda-promoter Geobacillus thermoleovorans

Inhibitors

Inhibitors Comment Organism Structure
Isopropanol slight inhibition at 1% Geobacillus thermoleovorans
additional information poor inhibition by Mg2+ at 1 mM, and by 2-mercaptoethanol, DTT, SDS, and EDTA at 1% Geobacillus thermoleovorans
Zn2+ 86% inhibition at 1 mM Geobacillus thermoleovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
4-nitrophenyl palmitate pH 7.5,60°C Geobacillus thermoleovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activation Geobacillus thermoleovorans
DMSO slight activation Geobacillus thermoleovorans
additional information no effect by Cs+ Geobacillus thermoleovorans
Na+ slight activation Geobacillus thermoleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 46000, pre-protein, SDS-PAGE, x * 43000, mature LipA, SDS-PAGE Geobacillus thermoleovorans
46000
-
x * 46000, pre-protein, SDS-PAGE, x * 43000, mature LipA, SDS-PAGE Geobacillus thermoleovorans

Organism

Organism UniProt Comment Textmining
Geobacillus thermoleovorans
-
isolated from desert soil in Egypt, gene lipA
-
Geobacillus thermoleovorans YN
-
isolated from desert soil in Egypt, gene lipA
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme contains a signal sequence of 28 amino acids Geobacillus thermoleovorans

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 130fold from Escherichia coli by immobilized metal-affinity chromatography and gel filtration Geobacillus thermoleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3586
-
purified recombinant lipase Geobacillus thermoleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
Geobacillus thermoleovorans 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Geobacillus thermoleovorans YN 4-nitrophenol + butyrate
-
?
4-nitrophenyl caprate + H2O best 4-nitrophenyl ester substrate Geobacillus thermoleovorans 4-nitrophenol + caprate
-
?
4-nitrophenyl caproate + H2O
-
Geobacillus thermoleovorans 4-nitrophenol + caproate
-
?
4-nitrophenyl caproate + H2O
-
Geobacillus thermoleovorans YN 4-nitrophenol + caproate
-
?
4-nitrophenyl caprylate + H2O
-
Geobacillus thermoleovorans 4-nitrophenol + caprylate
-
?
4-nitrophenyl caprylate + H2O
-
Geobacillus thermoleovorans YN 4-nitrophenol + caprylate
-
?
4-nitrophenyl laurate + H2O
-
Geobacillus thermoleovorans 4-nitrophenol + laurate
-
?
4-nitrophenyl myristate + H2O
-
Geobacillus thermoleovorans 4-nitrophenol + myristate
-
?
4-nitrophenyl palmitate + H2O
-
Geobacillus thermoleovorans 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O
-
Geobacillus thermoleovorans YN 4-nitrophenol + palmitate
-
?
4-nitrophenyl stearate + H2O
-
Geobacillus thermoleovorans 4-nitrophenol + stearate
-
?
additional information substrate specificity, methyl eicosanoate and methyl decosanoate are poor substrates, overview Geobacillus thermoleovorans ?
-
?
additional information substrate specificity, methyl eicosanoate and methyl decosanoate are poor substrates, overview Geobacillus thermoleovorans YN ?
-
?
triacetin + H2O
-
Geobacillus thermoleovorans diacetin + acetate
-
?
tributyrin + H2O
-
Geobacillus thermoleovorans dibutyrin + butyrate
-
?
tricaprylin + H2O best triacylglyceride substrate Geobacillus thermoleovorans dicaprylin + caprylate
-
?
trimyristin + H2O
-
Geobacillus thermoleovorans dimyristin + myristate
-
?
tripalmitin + H2O high activity Geobacillus thermoleovorans dipalmitin + palmitate
-
?

Subunits

Subunits Comment Organism
? x * 46000, pre-protein, SDS-PAGE, x * 43000, mature LipA, SDS-PAGE Geobacillus thermoleovorans

Synonyms

Synonyms Comment Organism
LipA
-
Geobacillus thermoleovorans
LipAB
-
Geobacillus thermoleovorans
lipase
-
Geobacillus thermoleovorans
lipase A
-
Geobacillus thermoleovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 65 recombinant enzyme Geobacillus thermoleovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
1 h, purified recombinant enzyme, stable up to Geobacillus thermoleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
recombinant enzyme Geobacillus thermoleovorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
purified recombinant enzyme, stable, at 60°C Geobacillus thermoleovorans
10.5
-
purified recombinant enzyme, stable, at 60°C Geobacillus thermoleovorans