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Literature summary for 3.1.1.3 extracted from
- Effect of nonionic surfactants on Rhizopus homothallicus lipase activity: a comparative kinetic study (2007), Mol. Biotechnol., 35, 205-214.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| taurodeoxycholate | 1.92fold activation, pH 7.5, 25°C | Rhizopus homothallicus |  |
| Triton X-100 | interacts with SSF and SmF lipases increasing their activity 1.7fold, pH 7.5, 25°C | Rhizopus homothallicus | |
| Tween 80 | 1.25fold activation, pH 7.5, 25°C | Rhizopus homothallicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyl 4-nitrophenyl phosphate | i.e. E600, 80% and 60% inhibition of SSF lipase and SmF lipase, respectively, pH 7.5, 25°C | Rhizopus homothallicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29500 | - |
SSF and SmF lipases, gel filtration | Rhizopus homothallicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus homothallicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SSF and SmF lipases by hydrophobic interaction chromatography and gel filtration | Rhizopus homothallicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | solid and submerged state fermentation methods | Rhizopus homothallicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8600 | - |
SmF lipase | Rhizopus homothallicus |
| 10700 | - |
SSF lipase | Rhizopus homothallicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trioctanoin + H2O | - |
Rhizopus homothallicus | dioctanoin + octanoate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Rhizopus homothallicus |
| SmF lipase | - |
Rhizopus homothallicus |
| SSF lipase | - |
Rhizopus homothallicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rhizopus homothallicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rhizopus homothallicus |
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Release 2023.1 (January 2023)
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