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Literature summary for 3.1.1.3 extracted from
- Purification and characterization of an alkaline lipase from Pseudomonas aeruginosa isolated from putrid mineral cutting oil as component of metalworking fluid (2006), J. Biosci. Bioeng., 102, 82-89.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| chloroform | at 25% v/v, 30°C, 130% activity after 24 h, 140% after 48 h | Pseudomonas aeruginosa |  |
| dimethylformamide | at 25% v/v, 30°C, 120% activity after 24 h, 110% after 48 h | Pseudomonas aeruginosa | |
| DTT | 20% activation at 5 mM | Pseudomonas aeruginosa | |
| EDTA | 30% activation at 5 mM | Pseudomonas aeruginosa | |
| iodoacetic acid | 10% activation at 5 mM | Pseudomonas aeruginosa | |
| sodium deoxycholate | 300% activation at 5 mM | Pseudomonas aeruginosa | |
| Triton X-100 | 300% activation at 5 mM | Pseudomonas aeruginosa | |
| Tween 80 | 200% activation at 5 mM | Pseudomonas aeruginosa | |
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | the alkaline lipase is stable and active in organic solvents and a water-restricted environment and has a great potential as a biotechnological tool e.g. in organosynthetic reactions in water-restricted medium or in control and prevention of metalworking fluid putrification in the metal industry | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 50% inhibition at 5 mM | Pseudomonas aeruginosa | |
| Cu2+ | 92% inhibition at 5 mM | Pseudomonas aeruginosa | |
| diisopropyl fluorophosphate | 30% inhibition at 5 mM, 60% inhibition at 25 mM | Pseudomonas aeruginosa | |
| Hg2+ | 94% inhibition at 5 mM | Pseudomonas aeruginosa | |
| Mg2+ | 32% inhibition at 5 mM | Pseudomonas aeruginosa | |
| Mn2+ | 47% inhibition at 5 mM | Pseudomonas aeruginosa | |
| additional information | no inhibition by EDTA, DTT, iodoacetic acid, and o-phenanthroline | Pseudomonas aeruginosa | |
| PMSF | 19% inhibition at 5 mM | Pseudomonas aeruginosa | |
| SDS | 93% inhibition at 5 mM | Pseudomonas aeruginosa | |
| Zn2+ | 85% inhibition at 5 mM | Pseudomonas aeruginosa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted to the culture medium | Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| triacylglycerol + H2O | Pseudomonas aeruginosa | - |
diacylglycerol + a carboxylate | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | at 25% v/v, 30°C, 95% remaining activity after 24 h, 33% after 48 h | Pseudomonas aeruginosa |
| Butanol | at 25% v/v, 30°C, 10% remaining activity after 48 h | Pseudomonas aeruginosa |
| chloroform | at 25% v/v, 30°C, 130% activity after 24 h, 140% after 48 h | Pseudomonas aeruginosa |
| dimethylformamide | at 25% v/v, 30°C, 120% activity after 24 h, 110% after 48 h, 100% remaining activity after 30 min at 50°C | Pseudomonas aeruginosa |
| Ethanol | at 25% v/v, 30°C, 90% remaining activity after 24 h, 17% after 48 h, 55% remaining activity after 10 min at 50°C | Pseudomonas aeruginosa |
| ethyl methyl ketone | 75% remaining activity after 10 min at 50°C | Pseudomonas aeruginosa |
| hexane | at 25% v/v, 30°C, 100% remaining activity after 24 h, 64% after 48 h, 100% reaming activity after 15 min at 60°C, 50% reamining activity after 30 min at 60°C | Pseudomonas aeruginosa |
| isopropanol | at 25% v/v, 30°C, 65% remaining activity after 24 h, 16% after 48 h | Pseudomonas aeruginosa |
| Methanol | at 25% v/v, 30°C, 80% remaining activity after 24 h, 42% after 48 h, 100% remaining activity after 10 min at 50°C | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
strain san-ai, isolated from putrid mineral cutting oil, with pH 10.0, as component of metalworking fluid for cooling and lubrication in industrial metalworking processes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 12.5fold from culture broth by ammonium sulfate fractionation, hydrophobic interaction chromatography, dialysis, and gel filtration to homogeneity | Pseudomonas aeruginosa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00006 | - |
purified enzyme, 95% acetone, 30°C | Pseudomonas aeruginosa |
| 0.00012 | - |
purified enzyme, 95% ethyl-methyl-ketone, 30°C | Pseudomonas aeruginosa |
| 0.00013 | - |
purified enzyme, 95% n-hexane, 30°C | Pseudomonas aeruginosa |
| 0.0052 | - |
purified enzyme, 95% ethanol, 30°C | Pseudomonas aeruginosa |
| 0.231 | - |
purified enzyme, 95% methanol, 30°C | Pseudomonas aeruginosa |
| 473 | - |
purified enzyme, buffer | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl palmitate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + palmitate | - |
? | |
| methyl oleate + H2O | acylglycerol lipase activity, EC 3.1.1.23 | Pseudomonas aeruginosa | methanol + oleate | - |
? | |
| triacylglycerol + H2O | - |
Pseudomonas aeruginosa | diacylglycerol + a carboxylate | - |
? | |
| triacylglycerol + H2O | the enzyme acts on triacylglycerols with medium fatty acid chain length, substrate specificity, overview | Pseudomonas aeruginosa | diacylglycerol + a carboxylate | - |
? | |
| tributyrin + H2O | - |
Pseudomonas aeruginosa | dibutyrin + butyrate | - |
? | |
| tricaprin + H2O | high activity | Pseudomonas aeruginosa | dicaprin + caprate | - |
? | |
| tricaproin + H2O | high activity | Pseudomonas aeruginosa | dicaproin + caproate | - |
? | |
| tricaprylin + H2O | best substrate | Pseudomonas aeruginosa | dicaprylin + caprylate | - |
? | |
| triolein + H2O | - |
Pseudomonas aeruginosa | diolein + oleate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline lipase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Pseudomonas aeruginosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 90 | - |
Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
15 min, purified native enzyme, 75% remaining activity | Pseudomonas aeruginosa |
| 70 | - |
15 min, purified native enzyme, 30% remaining activity | Pseudomonas aeruginosa |
| 80 | - |
complete inactivation within 10 min | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 11 | - |
- |
Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11.5 | activity range | Pseudomonas aeruginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11.5 | stable | Pseudomonas aeruginosa |
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