Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | ROL29 lipase shows the interfacial activation phenomenon when using tripropionin or vinyl propionate as substrates, while noninduced lipase ROLw does not | Rhizopus arrhizus |
Cloned (Comment) | Organism |
---|---|
ROLw, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha | Rhizopus arrhizus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tetrahydrolipstatin | inhibition of ROLw | Rhizopus arrhizus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion of noninduced lipase ROLw | Rhizopus arrhizus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, ROLw, SDS-PAGE | Rhizopus arrhizus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizopus arrhizus | Q2QFX1 | fragment; noninduced lipase ROLw | - |
Rhizopus arrhizus WPG | Q2QFX1 | fragment; noninduced lipase ROLw | - |
Purification (Comment) | Organism |
---|---|
native extracellular ROLw from fungal culture supernatant by gel filtration and cation exchange chromatography | Rhizopus arrhizus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the maximal production of lipase is obtained at pH 6.0 and 30°C | Rhizopus arrhizus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
900 | - |
recombinant noninduced lipase ROLw, substrate tributyrin | Rhizopus arrhizus |
3500 | - |
recombinant noninduced lipase ROLw, substrate olive oil | Rhizopus arrhizus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ROLw is unable to hydrolyse triacylglycerols in the presence of high concentration of bile salts, it is a serine enzyme | Rhizopus arrhizus | ? | - |
? | |
additional information | ROLw is unable to hydrolyse triacylglycerols in the presence of high concentration of bile salts, it is a serine enzyme | Rhizopus arrhizus WPG | ? | - |
? | |
olive oil + H2O | - |
Rhizopus arrhizus | ? | - |
? | |
olive oil + H2O | - |
Rhizopus arrhizus WPG | ? | - |
? | |
tributyrin + H2O | - |
Rhizopus arrhizus | dibutyrin + butyrate | - |
? | |
tributyrin + H2O | - |
Rhizopus arrhizus WPG | dibutyrin + butyrate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, ROLw, SDS-PAGE | Rhizopus arrhizus |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Rhizopus arrhizus |
noninduced lipase | - |
Rhizopus arrhizus |
ROL29 | - |
Rhizopus arrhizus |
ROLw | - |
Rhizopus arrhizus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
noninduced lipase ROLw | Rhizopus arrhizus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
noninduced lipase ROLw | Rhizopus arrhizus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | noninduced lipase ROLw, stable | Rhizopus arrhizus |