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Literature summary for 3.1.1.3 extracted from
- Catalytic properties of mycelium-bound lipases from Aspergillus niger MYA 135 (2007), Appl. Microbiol. Biotechnol., 76, 861-866.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | olive oil strongly induces the mycelium-bound enzyme | Aspergillus niger |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | 1 h, pH 7.0, 37°C, the mycelium-bound and constitutive enzymes are very stable in presence of ethanol | Aspergillus niger |
| Methanol | 1 h, pH 7.0, 37°C, the mycelium-bound and constitutive enzymes are very stable in presence of methanol | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
strain MYA 135, formerly strain 419, constitutive and inducible enzymes | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | mycelium-bound inducible enzyme | Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
transesterification activity, overview | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme also shows transesterification activity with 4-nitrophenyl palmitate ester substrates, overview | Aspergillus niger | ? | - |
? |  |
| olive oil + H2O | - |
Aspergillus niger | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mycelium-bound lipase | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 8 | 35 | inducible mycelium-bound enzyme in presence of olive oil | Aspergillus niger |
| 35 | 45 | optimum 2, constitutive enzyme | Aspergillus niger |
| 48 | - |
optimum 1, constitutive enzyme | Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 55 | the enzyme shows high activity of both enzyme forms within the range of 4°C to 8°C | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 55 | 1 h, the temperature stability of the mycelium-bound enzyme form is increased by induction with 2% olive oil, the enzyme is completely stable at 25-40°C, but loses 42-86% of its activity at 55°C and 4°C, respectively, overview | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6.5 | constitutive enzyme | Aspergillus niger |
| 4 | - |
optimum 1, inducible mycelium-bound enzyme | Aspergillus niger |
| 7 | - |
optimum 2, inducible mycelium-bound enzyme | Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 9 | activity range of both enzyme forms | Aspergillus niger |
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