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Literature summary for 3.1.1.3 extracted from

  • Lianghua, T.; Liming, X.; Min, S.; Huaying, G.
    Purification and application of a lipase from Penicillium expansum PED-03 (2007), Appl. Biochem. Biotechnol., 142, 194-199.
    View publication on PubMed
Search for more literature for 3.1.1.3

Application

Application Comment Organism
synthesis the enzyme shows potential in the production of optically pure ibuprofen Penicillium expansum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Penicillium expansum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 28000, SDS-PAGE Penicillium expansum

Organism

Organism UniProt Comment Textmining
Penicillium expansum Q7LST4 precursor; isolated from the waste of a rap oil manufactory in China
-
Penicillium expansum PED-03 Q7LST4 precursor; isolated from the waste of a rap oil manufactory in China
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular lipase 81.8fold from fermentation broth by anion exchange chromatography and gel filtration Penicillium expansum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Penicillium expansum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
85.94
-
 purified extracellular lipase Penicillium expansum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-ibuprofen + 1-propanol purified enzyme in a mixture of 1-propanol-isooctane in a ratio of 3:1, the conversion of racemic ibuprofen reaches 46% with very high enantioselectivity for production of the (S)-enantiomer Penicillium expansum (S)-ibuprofen n-propyl ester + (R)-ibuprofen
-
 ?
(R,S)-ibuprofen + 1-propanol purified enzyme in a mixture of 1-propanol-isooctane in a ratio of 3:1, the conversion of racemic ibuprofen reaches 46% with very high enantioselectivity for production of the (S)-enantiomer Penicillium expansum PED-03 (S)-ibuprofen n-propyl ester + (R)-ibuprofen
-
 ?
4-nitrophenyl palmitate + H2O
-
 Penicillium expansum 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl palmitate + H2O
-
 Penicillium expansum PED-03 4-nitrophenol + palmitate
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Penicillium expansum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Penicillium expansum
55
-
 conversion of racemic ibuprofen Penicillium expansum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Penicillium expansum