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Literature summary for 3.1.1.3 extracted from
- Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3 (2005), Protein Expr. Purif., 41, 38-44.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | slight inhibition | Weizmannia coagulans |  |
| Co2+ | complete inhibition | Weizmannia coagulans | |
| Mn2+ | complete inhibition | Weizmannia coagulans | |
| Zn2+ | complete inhibition | Weizmannia coagulans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Weizmannia coagulans | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | activates | Weizmannia coagulans | |
| Hg2+ | highly activating | Weizmannia coagulans | |
| K+ | activates | Weizmannia coagulans | |
| Mg2+ | activates | Weizmannia coagulans | |
| additional information | no effect by Na+ | Weizmannia coagulans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 31000, SDS-PAGE | Weizmannia coagulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Weizmannia coagulans | - |
- |
- |
| Weizmannia coagulans BTS-3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 40fold to homogeneity by ammonium sulfate fractionation and ion exchange chromatography | Weizmannia coagulans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | optimization of culture conditions, overview, optimal enzyme production at 55°C, pH 8.5 on mustard oil as carbon source and peptone/yeast extract, ratio 1:1, as nitrogen sources | Weizmannia coagulans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Weizmannia coagulans |
| 4.8 | - |
purified enzyme | Weizmannia coagulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Weizmannia coagulans | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Weizmannia coagulans BTS-3 | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl caprylate + H2O | - |
Weizmannia coagulans | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl caprylate + H2O | - |
Weizmannia coagulans BTS-3 | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl formate + H2O | - |
Weizmannia coagulans | 4-nitrophenol + formate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Weizmannia coagulans | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Weizmannia coagulans BTS-3 | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl palmitate + H2O | best substrate | Weizmannia coagulans | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | best substrate | Weizmannia coagulans BTS-3 | 4-nitrophenol + palmitate | - |
? | |
| additional information | substrate specificity, the enzyme shows preference for triacylglyceride substrates with long fatty acid chains | Weizmannia coagulans | ? | - |
? | |
| additional information | substrate specificity, the enzyme shows preference for triacylglyceride substrates with long fatty acid chains | Weizmannia coagulans BTS-3 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, SDS-PAGE | Weizmannia coagulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline lipase | - |
Weizmannia coagulans |
| lipase | - |
Weizmannia coagulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Weizmannia coagulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
half-life is 2 h | Weizmannia coagulans |
| 60 | - |
half-life is 30 min | Weizmannia coagulans |
| 70 | - |
purified enzyme, 75% remaining activity after 20 min | Weizmannia coagulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Weizmannia coagulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.3 | - |
Weizmannia coagulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10.5 | purified enzyme, stable | Weizmannia coagulans |
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