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Literature summary for 3.1.1.3 extracted from

  • Ogino, H.; Hiroshima, S.; Hirose, S.; Yasuda, M.; Ishimi, K.; Ishikawa, H.
    Cloning, expression and characterization of a lipase gene (lip3) from Pseudomonas aeruginosa LST-03 (2004), Mol. Genet. Genomics, 271, 189-196.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene lip3, shotgun cloning, construction of a genomic library, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 Pseudomonas aeruginosa

General Stability

General Stability Organism
purified recombinant enzyme, 30°C, pH 8.0, half-life is 12.3 days Pseudomonas aeruginosa

Organic Solvent Stability

Organic Solvent Comment Organism
1,4-butanediol half-life is 7.4 days Pseudomonas aeruginosa
1,5-pentanediol half-life is 0.19 days Pseudomonas aeruginosa
cyclohexane half-life is 0.48 days Pseudomonas aeruginosa
dimethyl sulfoxide half-life is 3.4 days Pseudomonas aeruginosa
Ethanol half-life is 0.20 days Pseudomonas aeruginosa
Ethylene glycol half-life is 84.6 days Pseudomonas aeruginosa
Glycerol half-life is 37 days Pseudomonas aeruginosa
Methanol half-life is 0.29 days Pseudomonas aeruginosa
additional information strain LST-03 is organic solvent-tolerant, overview Pseudomonas aeruginosa
N,N-dimethylformamide half-life is 7.9 days Pseudomonas aeruginosa
n-decane half-life is 0.40 days Pseudomonas aeruginosa
n-heptane half-life is 7.9 days Pseudomonas aeruginosa
n-hexane half-life is 7.7 days Pseudomonas aeruginosa
n-octane half-life is 9.2 days Pseudomonas aeruginosa
tert-Butanol half-life is 0.17 days Pseudomonas aeruginosa
toluene half-life is 0.37 days Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9KJG6 gene lip3
-
Pseudomonas aeruginosa LST-03 Q9KJG6 gene lip3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain JM109 by acetone fractionation, ion exchange and hydrophobic interaction chromatography to homogeneity Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
purified recombinant enzyme, 30°C, pH 8.0, half-life is 12.3 days Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dimercaptopropan-1-ol tributyrate + H2O
-
Pseudomonas aeruginosa ?
-
?
2,3-dimercaptopropan-1-ol tributyrate + H2O
-
Pseudomonas aeruginosa LST-03 ?
-
?
tributyrin + H2O
-
Pseudomonas aeruginosa dibutyrin + butyrate
-
?
tributyrin + H2O
-
Pseudomonas aeruginosa LST-03 dibutyrin + butyrate
-
?

Synonyms

Synonyms Comment Organism
lipase
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Pseudomonas aeruginosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 almost no activity above 50°C Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
stable up to Pseudomonas aeruginosa
45
-
inactivation above Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
4 8
-
Pseudomonas aeruginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 stable Pseudomonas aeruginosa