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Literature summary for 3.1.1.3 extracted from
- A tomato lipase homologous to DAD1 (LeLID1) is induced in post-germinative growing stage and encodes a triacylglycerol lipase (2004), FEBS Lett., 569, 195-200.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli, native enzyme partially by subcellular fractionation | Solanum lycopersicum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | no enzyme in the oil body fraction | Solanum lycopersicum | - |
- |
| soluble | - |
Solanum lycopersicum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47500 | - |
x * 47500, recombinant His-tagged enzyme, SDS-PAGE | Solanum lycopersicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Solanum lycopersicum | the enzyme probably is involved in fat mobilization during seed germination | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | - |
cv. Momotarou | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by Co2+ affinity chromatography | Solanum lycopersicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | high expression level | Solanum lycopersicum | - |
| flower | low expression level | Solanum lycopersicum | - |
| fruit | low expression level | Solanum lycopersicum | - |
| hypocotyl | - |
Solanum lycopersicum | - |
| additional information | no enzyme expression in root | Solanum lycopersicum | - |
| seed | high increase in enzyme expression during germination | Solanum lycopersicum | - |
| seedling | maximum activity 4 days after germination | Solanum lycopersicum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme probably is involved in fat mobilization during seed germination | Solanum lycopersicum | ? | - |
? | |
| additional information | substrate specificity, the enzyme catalyzes hydrolysis of triacylglycerides with long fatty acid chains, low activity with tripalmitin and tristearin, no activity with tributyrin and triacetate, phosphatidylcholine and monogalactosyldiacylglycerol are poor substrates | Solanum lycopersicum | ? | - |
? | |
| tricaprylin + H2O | - |
Solanum lycopersicum | dicaprylin + caprylate | - |
? | |
| triolein + H2O | - |
Solanum lycopersicum | diolein + oleate | - |
? | |
| tripalmitin + H2O | low activity | Solanum lycopersicum | dipalmitin + palmitate | - |
? | |
| tristearin + H2O | low activity | Solanum lycopersicum | distearin + stearate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47500, recombinant His-tagged enzyme, SDS-PAGE | Solanum lycopersicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L1D1 | - |
Solanum lycopersicum |
| lipase homologous to DAD1 | - |
Solanum lycopersicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Solanum lycopersicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant enzyme | Solanum lycopersicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity profile of the recombinant His-tagged enzyme | Solanum lycopersicum |
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Release 2023.1 (January 2023)
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