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Literature summary for 3.1.1.3 extracted from

  • Fahmy, A.S.; Abdel-Gany, S.S.; Mohamed, T.M.; Mohamed, S.A.
    Esterase and lipase in camel tick Hyalomma dromedarii (Acari: Ixodidae) during embryogenesis (2004), Comp. Biochem. Physiol. B, 137B, 159-168.
No PubMed abstract available
Search for more literature for 3.1.1.3

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxymercuribenzoate competitive inhibition of LIII, 60% inhibition at 5 mM Hyalomma dromedarii
Ca2+ 50% inhibition at 1 mM Hyalomma dromedarii
DTNB competitive inhibition of LIII, 30% inhibition at 5 mM Hyalomma dromedarii
Fe3+ 68% inhibition at 1 mM Hyalomma dromedarii
Hg2+ 75% inhibition at 1 mM Hyalomma dromedarii
Li+ 56% inhibition at 1 mM Hyalomma dromedarii
Mn2+ 81% inhibition at 1 mM Hyalomma dromedarii
additional information no inhibition by 2-mercaptoethanol, PMSF, and iodoacetamide Hyalomma dromedarii
NEM 10% inhibition at 5 mM Hyalomma dromedarii
Ni2+ 86% inhibition at 1 mM Hyalomma dromedarii
Zn2+ 98% inhibition at 1 mM Hyalomma dromedarii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Hyalomma dromedarii

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not affected by Mg2+ at 1 mM Hyalomma dromedarii

Organism

Organism UniProt Comment Textmining
Hyalomma dromedarii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by ion exchange chromatography, separation from esterases Hyalomma dromedarii

Source Tissue

Source Tissue Comment Organism Textmining
embryo the enzyme activity changes during embryogenesis Hyalomma dromedarii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.063
-
 partially purified enzyme Hyalomma dromedarii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
triolein + H2O olive oil Hyalomma dromedarii diolein + oleate
-
 ?

Synonyms

Synonyms Comment Organism
lipase
-
 Hyalomma dromedarii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Hyalomma dromedarii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 loss of 53% activity after 15 min Hyalomma dromedarii
90
-
 15 min, inactivation Hyalomma dromedarii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Hyalomma dromedarii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 4-hydroxymercuribenzoate pH 8.0, 37°C Hyalomma dromedarii
0.25
-
 DTNB pH 8.0, 37°C Hyalomma dromedarii